CORE--ANALYTICAL AND APPLIED NEUROSCIENCE

分析与应用神经科学(AAN)的首要目标 核心将成为(在技术上和理论上) 临床假说与基础神经科学与分析 测试所需的参数。因此，AAN核心提供 MHCRC科学家，对任何分析物(内源性或 外来生物)，这将有助于进行经批准的研究或 制定临床前研究战略，以解决 调查者的假设。此外，该核心还提供这些服务 与咨询有关的优势和限制 这样的方法和策略才能达到预期的研究目的。这些服务 提供的是具有成本效益的，并且通常代表 调查员要么不能轻易获得，要么只能通过 极大的困难或费用。核心使用不同的方法 [从放射免疫分析和高效液体 高效液相色谱-电喷雾-质谱法 (HPLC-ESMS)]对这些分子进行量化，这些分子对 检验科学假说，包括临床和基础。如上所述 如上所述，不仅核心实现或发展必要 技术和执行分析，但它也提供咨询，以 与研究设计相关的临床研究人员(例如，措施的效用， 样品的采集和储存、解释等)。最后，有了 MHCRC科学审查委员会的批准，这一核心 为与MHCRC相关的客户提供分析服务和协作 基础科学家，并通过这样做，巩固了临床之间的重要联系 基础科学家和他们的思想交流。它的范围是 由核心提供的服务导致了它现在的名字。其核心是 还为基本的MHCRC提供技术援助和资源 科学家为他们的工作所需的程序和服务，但 他们既没有足够的专业知识，也没有足够的手段。解决问题的关键 实现这些目标的能力是该核心提供状态 内源分子和外源物质的最新分析方法 (及其代谢物)。在过去的五年中，MHCRC已经 采取多个步骤最大限度地提高效率和响应速度 实现这些目标。然而，必须指出的是，AAN核心 是一个单一的单位，提供以前属于 三个独立的基于实验室的岩心，已在 最后一次竞争的申请(见下文)。背景和理论基础 中详细讨论了组织结构的这一重大变化 C.1节。

The primary goal of the Analytical and Applied Neuroscience (AAN) Core is to be the bridge (both technically and theoretically) between clinical hypotheses and the basic neuroscience and analytical parameters required for their testing. Thus, the AAN Core provides MHCRC scientist with quantification of any analyte (endogenous or exonobiotic) that will assist in conducting an approved study or the development of a preclinical research strategy that will address the investigator's hypotheses. Moreover, this Core provides these services in conjunction with consultation about the strengths and limitations of such methods and strategies for the desired studies. The services provided are cost-efficient, and often represent assistance that the investigator either could not readily obtain, or could obtain only with extreme difficulty or expense. The core uses diverse methodologies [ranging from radioimmunoassays and High Performance Liquid Chromatography (HPLC), to HPLC-electrospray mass spectrometry (HPLC-ESMS)] to quantify such molecules that are critical to the testing of scientific hypotheses, both clinical and basic. As noted above, not only does the core implement or develop the necessary techniques and perform the assays, but it also provides consultation to clinical investigators related to study design (e.g., utility of measures, sample acquisition and storage, interpretation, etc.). Finally, with approval of the MHCRC Scientific Review Committee, this core provides analytical service and collaboration to MHCRC-associated basic scientists, and by so doing, solidifies the vital link between clinic and basic scientists and their exchange of ideas. It is the range of the services provided by the core that led to its present name. The core also provides technical assistance and resources to basic MHCRC scientists for procedures and services that their work requires, but for which they have neither sufficient expertise nor means. The key to accomplishing these ends is the ability of this core to provide state-of- the-art analytical assays of both endogenous molecules and xenobiotics (and their metabolites). During the past five years, the MHCRC has taken several steps to maximize the efficiency and responsiveness of achieving these ends. Yet it is imperative to note that the AAN Core is a single unit that provides services that were formerly the domain of three separate laboratory-based cores which had been approved in the last competing application (see below). The background and rationale for this significant change in organization are discussed in detail in Section C.1.