CORE--ANALYTICAL AND APPLIED NEUROSCIENCE

核心——分析和应用神经科学

• 批准号: 6496357
• 负责人: Richard B Mailman
• 金额: $ 26.22万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-01 至 2002-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6496357
• 关键词: analytical chemistry; analytical method; autoradiography; behavior disorders; biomedical facility; electrospray ionization mass spectrometry; experimental designs; gas chromatography; gene targeting; high performance liquid chromatography; in situ hybridization; mental disorders; method development; neuroendocrine system; neurosciences; pharmacokinetics; psychopharmacology; radioimmunoassay

The primary goal of the Analytical and Applied Neuroscience (AAN) Core is to be the bridge (both technically and theoretically) between clinical hypotheses and the basic neuroscience and analytical parameters required for their testing. Thus, the AAN Core provides MHCRC scientist with quantification of any analyte (endogenous or exonobiotic) that will assist in conducting an approved study or the development of a preclinical research strategy that will address the investigator's hypotheses. Moreover, this Core provides these services in conjunction with consultation about the strengths and limitations of such methods and strategies for the desired studies. The services provided are cost-efficient, and often represent assistance that the investigator either could not readily obtain, or could obtain only with extreme difficulty or expense. The core uses diverse methodologies [ranging from radioimmunoassays and High Performance Liquid Chromatography (HPLC), to HPLC-electrospray mass spectrometry (HPLC-ESMS)] to quantify such molecules that are critical to the testing of scientific hypotheses, both clinical and basic. As noted above, not only does the core implement or develop the necessary techniques and perform the assays, but it also provides consultation to clinical investigators related to study design (e.g., utility of measures, sample acquisition and storage, interpretation, etc.). Finally, with approval of the MHCRC Scientific Review Committee, this core provides analytical service and collaboration to MHCRC-associated basic scientists, and by so doing, solidifies the vital link between clinic and basic scientists and their exchange of ideas. It is the range of the services provided by the core that led to its present name. The core also provides technical assistance and resources to basic MHCRC scientists for procedures and services that their work requires, but for which they have neither sufficient expertise nor means. The key to accomplishing these ends is the ability of this core to provide state-of- the-art analytical assays of both endogenous molecules and xenobiotics (and their metabolites). During the past five years, the MHCRC has taken several steps to maximize the efficiency and responsiveness of achieving these ends. Yet it is imperative to note that the AAN Core is a single unit that provides services that were formerly the domain of three separate laboratory-based cores which had been approved in the last competing application (see below). The background and rationale for this significant change in organization are discussed in detail in Section C.1.

分析与应用神经科学（AAN） 核心是成为（技术上和理论上） 临床假设和基础神经科学以及分析 测试所需的参数。 因此，AAN核心提供 MHCRC科学家对任何分析物（内源性或 外生生物），这将有助于进行批准的研究或 制定临床前研究策略， 调查员的假设 此外，该核心还提供这些服务 与协商的优势和局限性， 这种方法和策略，为所需的研究。 的服务 提供的援助具有成本效益，往往是 研究者不能轻易获得，或只能获得 极端的困难或花费。 核心使用不同的方法 [包括放射免疫测定和高效液相色谱法， 色谱法（HPLC）至HPLC-电喷雾质谱法 （HPLC-ESMS）]来定量对细胞生长至关重要的分子。 检验科学假设，包括临床和基础。 如所指出 以上，不仅核心实施或开发必要的 技术和执行分析，但它也提供咨询， 与研究设计相关的临床研究者（例如，措施的效用， 样品采集和存储、解释等）。 最后以 MHCRC科学审查委员会的批准，该核心 提供分析服务和协作， 基础科学家，并通过这样做，巩固了临床之间的重要联系， 和基础科学家以及他们的思想交流。 这是一个范围的 核心提供的服务，导致其目前的名称。 核心 还为基本的MHCRC提供技术援助和资源 科学家的程序和服务，他们的工作需要，但对于 他们既没有足够的专业知识，也没有足够的手段。 的关键 实现这些目标的能力是这个核心提供国家的， 内源性分子和外源性物质的现有技术分析测定 (and其代谢物）。 在过去五年中，MHCRC 采取了几个步骤，以最大限度地提高效率和反应能力， 实现这些目标。 然而，必须指出的是，AAN核心 是一个单一的单位，提供服务，以前是域 三个独立的实验室核心， 最后一个竞争申请（见下文）。背景和理由 中详细讨论了这一重大变化 第C.1节。