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ELECTRICALLY EVOKED OTOACOUSTIC EMISSION

电诱发耳声发射

基本信息

批准号：
6379417
负责人：
TIANYING REN
金额：
$ 5.26万
依托单位：
OREGON HEALTH & SCIENCE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1999
资助国家：
美国
起止时间：
1999-08-01 至 2002-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6379417
关键词：
auditory feedback cochlea ear hair cell electrodes electrostimulus evoked potentials furosemide gerbil /jird noise otoacoustic emission postmortem sound perception time perception

项目摘要

For the perception of complex environmental sounds, the normal mammalian ear has an extremely wide dynamic range and high frequency selectivity. These auditory capabilities are achieved by cochlear high sensitivity, nonlinearity and sharp tuning. A cochlear amplifier has been proposed to amplify the basilarmembrane vibration evoked by low level sound and enhance mechanical frequency selectivity. A widely accepted speculation is that the amplifier works by obtaining energy from the outer hair cells (OHCs). Although isolated OHC electromotility in vitro has been intensively studied and the cochlear amplifier theory is generally accepted, the role of the OHC electromotility in the cochlear amplifier has not been tested in vivo and in sensitive cochleae. The long-term objective of the proposed work is to determine whether a cochlear amplifier feedback system utilizes OHC-generated energy. In order to achieve the long-term goal, it is essential to characterize the electrically evoked otoacoustic emissions (EEOAE). The EEOAE will be investigated in this initial study by focusing on the fine structure of the EEOAE. Our working hypothesis is that the ear canal-measured EEOAE is a vector summation of the multicomponents of the electrically evoked mechanical response in the cochlea. The fine structure of the EEOAE results from the cancellation and enhancement of the different components, and the fine structure indicates normal mechanical properties of a sensitive cochlea. To preserve cochlear sensitivity, an extracochlear electrical stimulation animal model will be used to generate the EEOAE. A method for detecting multicomponents of a single frequency signal will be implemented to analyze the EEOAE. The specific aims of the proposed study are to investigate i) how the fine structure of the EEOAE is generated; ii) what the relationship is between the EEOAE fine structure and cochlear sensitivity; and iii) what the correspondence is between the EEOAE and acoustically evoked cubic distortion product otoacoustic emission (DPOAE) fine structures. The responses of each of the components of the EEOAE to cochlear sensitivity changes and a simultaneously presented acoustic stimulus will be observed. Similarity of the multiple components between the EEOAE and the DPOAE will also be studied. Results from the proposed experiments will provide evidence that the fine structure results from the interaction of the multicomponents of the EEOAE, and that the multicomponent analysis method is a reliable method for quantification of the fine structure of the otoacoustic emissions.

对于复杂环境声音的感知，正常哺乳动物的耳朵具有极宽的动态范围和高频选择性。这些听觉能力是通过耳蜗的高灵敏度、非线性和敏锐调谐来实现的。已提出耳蜗放大器来放大低声级声音引起的基底膜振动并增强机械频率选择性。一种广泛接受的推测是放大器通过从外毛细胞（OHC）获取能量来工作。尽管体外分离的 OHC 电动性已得到深入研究，并且耳蜗放大器理论已被普遍接受，但 OHC 电动性在耳蜗放大器中的作用尚未在体内和敏感耳蜗中进行测试。这项工作的长期目标是确定耳蜗放大器反馈系统是否利用 OHC 生成的能量。为了实现长期目标，有必要表征电诱发耳声发射（EEOAE）。在这项初步研究中，我们将重点关注 EEOAE 的精细结构来研究 EEOAE。我们的工作假设是，耳道测量的 EEOAE 是耳蜗中电诱发机械响应的多个分量的矢量求和。 EEOAE 的精细结构是由不同成分的抵消和增强产生的，并且精细结构表明敏感耳蜗的正常机械特性。为了保持耳蜗敏感性，将使用耳蜗外电刺激动物模型来生成 EEOAE。将采用一种检测单频信号多分量的方法来分析 EEOAE。本研究的具体目标是研究 i) EEOAE 的精细结构是如何生成的； ii) EEOAE 精细结构与耳蜗敏感性之间的关系是什么； iii) EEOAE 和声诱发立方畸变产物耳声发射 (DPOAE) 精细结构之间的对应关系是什么。将观察 EEOAE 的每个组成部分对耳蜗敏感性变化和同时呈现的声刺激的响应。还将研究 EEOAE 和 DPOAE 之间多个组件的相似性。所提出的实验结果将提供证据证明精细结构是 EEOAE 多组分相互作用的结果，并且多组分分析方法是量化耳声发射精细结构的可靠方法。