Genetic and Epigenetic Studies of Aging

描述(来自应用程序)：此操作的长期目标 计划项目是了解参与的基本分子机制 人类细胞衰老。具体目标是：1)汇聚六个 使用不同细胞衰老模型系统的各个实验室： 正常人成纤维细胞、肾上腺皮质细胞和黑素细胞 永生的人类细胞系和动物模型；共同研究类似的 目标，使用通用技术和方法回答以下问题 衰老和衰老的分子和细胞机制 永垂不朽。这些研究将检验基因表达的变化。 当细胞在体外和体内老化时发生的，并将建立 导致细胞抑制的途径的异同 扩散。认识到老龄化是一个多方面的过程， 个别项目将探索与年龄相关的变化的各种机制 基因表达，包括DNA甲基化、染色结构、衰老 特异性反式激活因子和RNA结合蛋白及其相关性 从体外到体内老化。2)提供以下所需的核心设施 所有项目，从而整合资源，使 以低得多的成本进行研究。因此，计划项目的目标将是 MET，鼓励成功的互动并加强对 公共资源。3)提供促进研究的正式机制 通过联合规划会议进行互动。

DESCRIPTION (from the application): The long range goal of this program project is to understand the basic molecular mechanisms involved in human cellular senescence. The specific aims are to: 1) Bring together six individual laboratories using disparate model systems of cellular senescence: normal human fibroblasts, adrenocortical cells and melanocytes, various immortal human cell lines and animal models; to work together on similar goals, using common techniques and approaches to answer questions regarding the molecular and cellular mechanisms involved in senescence and immortalization. These studies will examine the changes in gene expression that occur in cells as they age in vitro and in vivo, and will establish the differences and similarities in the pathways that lead to inhibition of cell proliferation. Recognizing that aging is a multi-faceted process, the individual projects will explore various mechanisms of age related changes in gene expression, including DNA methylation, chromatic structure, senescence specific transactivating factors and RNA binding proteins and the relevance of in vitro to in vivo aging. 2) Provide core facilities that are requisite for all the projects and thereby consolidate resources that will benefit the research at a much lower cost. The aims of a program project will thereby be met, encouraging successful interactions and enhancing the utilization of common resources. 3) Provide a formal mechanism to promote research interactions via joint planning meetings.