Genetic and Epigenetic Studies of Aging

衰老的遗传和表观遗传研究

• 批准号: 6495244
• 负责人: JAMES SMITH
• 金额: $ 14.37万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCIENCE CENTER
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-01 至 2006-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6495244
• 关键词: aging; cell senescence; gene expression

DESCRIPTION (from the application): The long range goal of this program project is to understand the basic molecular mechanisms involved in human cellular senescence. The specific aims are to: 1) Bring together six individual laboratories using disparate model systems of cellular senescence: normal human fibroblasts, adrenocortical cells and melanocytes, various immortal human cell lines and animal models; to work together on similar goals, using common techniques and approaches to answer questions regarding the molecular and cellular mechanisms involved in senescence and immortalization. These studies will examine the changes in gene expression that occur in cells as they age in vitro and in vivo, and will establish the differences and similarities in the pathways that lead to inhibition of cell proliferation. Recognizing that aging is a multi-faceted process, the individual projects will explore various mechanisms of age related changes in gene expression, including DNA methylation, chromatic structure, senescence specific transactivating factors and RNA binding proteins and the relevance of in vitro to in vivo aging. 2) Provide core facilities that are requisite for all the projects and thereby consolidate resources that will benefit the research at a much lower cost. The aims of a program project will thereby be met, encouraging successful interactions and enhancing the utilization of common resources. 3) Provide a formal mechanism to promote research interactions via joint planning meetings.

描述（来自应用程序）：此项目的长期目标 计划项目是了解所涉及的基本分子机制 人体细胞衰老。具体目标是： 1）汇集六 使用不同的细胞衰老模型系统的各个实验室： 正常人成纤维细胞、肾上腺皮质细胞和黑素细胞，各种 永生人类细胞系和动物模型；共同致力于类似的工作 目标，使用常见的技术和方法来回答有关的问题 衰老相关的分子和细胞机制 永生化。这些研究将检查基因表达的变化 当细胞在体外和体内老化时，会发生这些变化，并将建立 导致细胞抑制的途径的差异和相似之处 增殖。认识到衰老是一个多方面的过程， 各个项目将探索与年龄相关的变化的各种机制 基因表达，包括DNA甲基化、染色结构、衰老 特异性反式激活因子和 RNA 结合蛋白及其相关性 体外到体内的老化。 2）提供必要的核心设施 所有项目，从而整合资源，使之受益 研究成本要低得多。因此，计划项目的目标将是 会面，鼓励成功的互动并提高资源的利用率 共同资源。 3）提供正式的机制来促进研究 通过联合规划会议进行互动。