GENETIC AND EPIGENETIC STUDIES OF AGING

衰老的遗传和表观遗传研究

• 批准号: 7349803
• 负责人: JAMES SMITH
• 金额: $ 3.85万
• 依托单位: TEXAS BIOMEDICAL RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7349803
• 关键词: GENETIC; EPIGENETIC; STUDIES; AGING

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The long range goal of this program project is to understand the basic molecular mechanisms involved in human cellular senescence. The specific aims are to: 1) Bring together six individual laboratories using disparate model systems of cellular senescence: normal human fibroblasts, adrenocortical cells and melanocytes, various immortal human cell lines and animal models; to work together on similar goals, using common techniques and approaches to answer questions regarding the molecular and cellular mechanisms involved in senescence and immortalization. These studies will examine the changes in gene expression that occur in cells as they age in vitro and in vivo, and will establish the differences and similarities in the pathways that lead to inhibition of cell proliferation. Recognizing that aging is a multi-faceted process, the individual projects will explore various mechanisms of age related changes in gene expression, including DNA methylation, chromatic structure, senescence specific transactivating factors and RNA binding proteins and the relevance of in vitro to in vivo aging. 2) Provide core facilities that are requisite for all the projects and thereby consolidate resources that will benefit the research at a much lower cost. The aims of a program project will thereby be met, encouraging successful interactions and enhancing the utilization of common resources. 3) Provide a formal mechanism to promote research interactions via joint planning meetings.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。这个项目的长期目标是了解人类细胞衰老的基本分子机制。具体目标是：1）将六个使用不同细胞衰老模型系统的实验室聚集在一起：正常人类成纤维细胞，肾上腺皮质细胞和黑色素细胞，各种永生人类细胞系和动物模型;共同致力于类似的目标，使用共同的技术和方法来回答有关衰老和永生化的分子和细胞机制的问题。这些研究将检查细胞在体外和体内老化时发生的基因表达变化，并将确定导致细胞增殖抑制的途径的差异和相似性。认识到衰老是一个多方面的过程，个别项目将探索与年龄相关的基因表达变化的各种机制，包括DNA甲基化，染色质结构，衰老特异性反式激活因子和RNA结合蛋白，以及体外与体内衰老的相关性。2)提供所有项目所需的核心设施，从而整合资源，以更低的成本使研究受益。因此，一个计划项目的目标将得到满足，鼓励成功的互动和提高共同资源的利用。3)通过联合规划会议，提供促进研究互动的正式机制。