NIH MACAQUE RESOURCES--DEFINITION OF HUMORAL IMMUNITY

这项提议的主要目标是进一步描述和描述 定义恒河猴和猪尾猕猴的体液免疫系统。 这些物种是一种正在使用的无价和独特的资源 随着与发病机制相关的研究的频率增加， 预防、诊断和治疗各种人类疾病。 恒河猴和猪尾猕猴是 美国国立卫生研究院区域灵长类研究中心项目遍布全国 对该中心的艾滋病毒/SIV研究和许多 其他由NIH资助的机构。尽管细胞免疫反应 在猕猴身上有很好的特征，有限的信息是 对它们的体液免疫有帮助。准确地定义了 免疫系统的体液臂，特别是和抗体 为了验证猕猴模型，曲目是必不可少的。至 定义这些非人类灵长类物种的体液免疫系统， 我们将在两个不同的水平上分析猕猴抗体谱， 遗传型表型。具体地说，提出了三个具体目标 目的：1)鉴定免疫球蛋白可变区基因及其产物 恒河猴和猪尾猕猴；2)鉴定免疫球蛋白常数 恒河猴和猪尾猕猴的区域基因及其产物；以及 3)研制针对猕猴免疫球蛋白变量的特异性试剂， 恒定区，并利用这些试剂定义抗体模式 在正常恒河猴和猪尾猕猴身上。实现这些目标 特定的目标将提供定义良好的非人类灵长类动物模型 了解抗体介导的保护性和致病性 新药的药效评价及作用机制 免疫干预策略。

The major objective of this proposal is to characterize further and define the humoral immune system of rhesus and pig-tailed macaques. These species are an invaluable and unique resource that are being used with increasing frequency for studies related to the pathogenesis, prophylaxis, diagnosis and therapy of a variety of human diseases. Rhesus and pig-tailed macaques are an important component of the NIH Regional Primate Research Center programs across the country and are essential to the HIV/SIV studies at the Centers and at numerous other NIH-funded institutions. Although cellular immune responses have been well characterized in macaques, limited information is available on their humoral immunity. The accurate definition of the humoral arm of the immune system, particularly and antibody repertoire, is imperative in order to validate the macaque model. To define the humoral immune system of these nonhuman primate species, we will analyze the macaque antibody repertoire at two different levels, genotypic phenotypic. Specifically, three specific aims are proposed to: 1) identify immunglobulin variable region genes and their products in rhesus and pig-tailed macaques; 2) identify immunoglobulin constant region genes and their products in rhesus and pig-tailed macaques; and 3) generate reagents specific for macaque immunoglobulin variable and constant regions and utilize these reagents to define antibody patterns in normal rhesus and pig-tailed macaques. Accomplishment of these specific aims will provide well-defined nonhuman primate models for understanding antibody-mediated protective and pathogenetic mechanisms and for the evaluation of the efficacy of novel immunointervention strategies.