NIH MACAQUE RESOURCES--DEFINITION OF HUMORAL IMMUNITY

这项建议的主要目的是进一步描述和 定义恒河猴和猪尾猕猴的体液免疫系统。 这些物种是一种宝贵而独特的资源， 随着与发病机制相关的研究频率的增加， 预防、诊断和治疗多种人类疾病。 恒河猴和猪尾猕猴是 NIH地区灵长类动物研究中心在全国各地的项目 并且对于中心和许多研究中心的HIV/SIV研究至关重要。 其他NIH资助的机构。 虽然细胞免疫反应 已经在猕猴中得到了很好的描述，有限的信息是 他们的体液免疫力。 准确的定义 免疫系统的体液分支，特别是抗体 为了验证猕猴模型， 到 定义这些非人类灵长类物种的体液免疫系统， 我们将在两个不同的水平上分析猕猴抗体库， 基因型表型 具体而言，提出了三个具体目标 目的：1）鉴定免疫球蛋白可变区基因及其产物 在恒河猴和猪尾猕猴中; 2）鉴定免疫球蛋白恒定区 恒河猴和猪尾猕猴的区域基因及其产物;以及 3)产生特异于猕猴免疫球蛋白可变区的试剂， 恒定区，并利用这些试剂来定义抗体模式 在正常的恒河猴和猪尾猕猴身上。 完成这些 具体的目标将提供明确的非人灵长类动物模型， 了解抗体介导的保护和致病 机制和评价新的 免疫干预策略。

The major objective of this proposal is to characterize further and define the humoral immune system of rhesus and pig-tailed macaques. These species are an invaluable and unique resource that are being used with increasing frequency for studies related to the pathogenesis, prophylaxis, diagnosis and therapy of a variety of human diseases. Rhesus and pig-tailed macaques are an important component of the NIH Regional Primate Research Center programs across the country and are essential to the HIV/SIV studies at the Centers and at numerous other NIH-funded institutions. Although cellular immune responses have been well characterized in macaques, limited information is available on their humoral immunity. The accurate definition of the humoral arm of the immune system, particularly and antibody repertoire, is imperative in order to validate the macaque model. To define the humoral immune system of these nonhuman primate species, we will analyze the macaque antibody repertoire at two different levels, genotypic phenotypic. Specifically, three specific aims are proposed to: 1) identify immunglobulin variable region genes and their products in rhesus and pig-tailed macaques; 2) identify immunoglobulin constant region genes and their products in rhesus and pig-tailed macaques; and 3) generate reagents specific for macaque immunoglobulin variable and constant regions and utilize these reagents to define antibody patterns in normal rhesus and pig-tailed macaques. Accomplishment of these specific aims will provide well-defined nonhuman primate models for understanding antibody-mediated protective and pathogenetic mechanisms and for the evaluation of the efficacy of novel immunointervention strategies.