MR STUDY OF BRAIN INTERSTITIAL WATER MOTION

描述（改编自申请人的摘要）：脑水运动， 可以通过MR技术检测到，取决于许多因素，一些 具有正常生理学特征和病理学特征。 因此，在本发明中， 扩散加权成像广泛用于评估许多 CNS表示。 水运动的维持本身对于 正常的脑功能，或者至少是一个很好的生理指标， 状态 尼尔/阿克曼实验室的研究项目主要集中在 阐明了决定水运动的因素。 该提案重点 了解更多关于细胞外水对总的 （细胞内加细胞外）水ADC。 组织液的运动 大量脑脊液（CSF）流量的贡献，房室 边界和布朗运动 设计实验以确定 这三个因素对ADC的相对贡献。 的间隙水 ADC将通过测量宽范围的运动来间接测量。 细胞外标志物。 最近的研究结果表明，化合物的ADC局限于 与细胞外间隙大致相等， 分子量（MW）的差异。 这表明大量脑脊液流动是一种 主导因素 这个想法将被测试与两个大的细胞外 标记物（MW=0.9和4 kD的聚乙二醇）和一个非常小的标记物 （钠，MW=23）。 最后，脑脊液流量将被重新改变， 直接评估其对细胞外水ADC的影响。

DESCRIPTION (Adapted from applicant's abstract): Brain water motion, which can be detected by MR techniques, varies depending on many factors, some characteristic of normal physiology and others of pathology. As a result, diffusion weighted imaging is used widely for the evaluation of a number of CNS states. Maintenance of water motion is either itself important for normal brain function or is at least an excellent marker of physiologic state. The Neil/Ackerman laboratory's research program focuses on elucidating the factors which determine water motion. This proposal focuses on learning more about the contribution of extracellular water to the total (intra- plus extracellular) water ADC. Motion in the interstitial fluid has contributions from bulk cerebrospinal fluid (CSF) flow, compartmental boundaries, and Brownian motion. Experiments were designed to determine the relative contribution of these three factors to ADC. The interstitial water ADC will be measured indirectly by measuring the motion of a broad range of extracellular markers. Recent results show that ADCs of compounds confined to the extracellular space are approximately equal despite marked differences in molecular weight (MW). This suggests that bulk CSF flow is a dominating factor. This idea will be tested with both large extracellular markers (polyethyleneglycols of MW=0.9 and 4 kD) and one very small marker (sodium, MW=23). Finally, CSF flow will be pharmacologically altered to directly assess its effect on extracellular water ADC.