An integrated approach to understanding mucosal immunity to Campylobacter infection in the chicken

了解鸡对弯曲杆菌感染的粘膜免疫的综合方法

• 批准号: 1644280
• 金额: --
• 依托单位: University of Liverpool
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2015
• 资助国家: 英国
• 起止时间: 2015 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1644280
• 关键词: integrated; approach; understanding; mucosal; immunity

This project uses a new way of working, by combining modelling and experimental approaches together in a way that both informs each arm of the project, but more importantly brings them together allowing us to better understand the complex interactions of the mucosal immune response to the bacterial pathogen Campylobacter jejuni. C. jejuni is the most common cause of foodborne bacterial gastroenteritis worldwide, with the overwhelming majority of cases associated with chicken. Despite the fact that around 70% of UK retail chicken is contaminated with Campylobacter our understanding of its infection biology in the broiler chicken in surprisingly limited. Our recent work has begun to elucidate the innate immune response to infection of the chicken and combining data from these studies with a modelling approach through Bayesian structural equation modelling has allowed us to identify immunological differences between chicken breeds that underlie differences in infection phenotype. This project will build upon previous work to model innate, adaptive and regulatory responses during C. jejuni infection to determine which are involved in long-term colonization of the chicken, those associated with clearance of infection and those with poor gut health. Using this combined approach gives us a unique insight as to what is important and how this could be utilized in controls such as vaccines or make improvements to chicken gut health thereby improving animal health and welfare and ultimately reduce the burden of Campylobacter associated with chicken production.

该项目使用了一种新的工作方式，通过将建模和实验方法结合在一起，既为项目的每个分支提供信息，更重要的是将它们结合在一起，使我们能够更好地了解粘膜免疫反应对细菌病原体空肠弯曲菌的复杂相互作用。C.空肠炎是全球最常见的食源性细菌性肠胃炎，绝大多数病例与鸡肉有关。尽管事实上，大约70%的英国零售鸡肉被弯曲杆菌污染，但我们对其在肉鸡中的感染生物学的了解令人惊讶地有限。我们最近的工作已经开始阐明鸡感染的先天免疫反应，并通过贝叶斯结构方程建模将这些研究的数据与建模方法相结合，使我们能够确定鸡品种之间的免疫差异，这些差异是感染表型差异的基础。这个项目将建立在以前的工作，以模拟先天，适应性和调节反应，在C。空肠感染，以确定哪些参与鸡的长期定殖，哪些与感染清除相关，哪些肠道健康状况较差。使用这种组合方法使我们对什么是重要的以及如何将其用于疫苗等控制或改善鸡肠道健康从而改善动物健康和福利并最终减少与鸡生产相关的弯曲杆菌负担有了独特的见解。

项目成果

B lymphocytes play a limited role in clearance of Campylobacter jejuni from the chicken intestinal tract.

• DOI: 10.1038/srep45090
• 发表时间: 2017-03-23
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Lacharme-Lora L;Chaloner G;Gilroy R;Humphrey S;Gibbs K;Jopson S;Wright E;Reid W;Ketley J;Humphrey T;Williams N;Rushton S;Wigley P
• 通讯作者: Wigley P