NONDIAMAGNETIC AGENTS IN IN VIVO 23 NA AND 1H20 MR

非磁性剂体内 23 NA 和 1H20 MR

• 批准号: 6385488
• 负责人: CHARLES S. SPRINGER
• 金额: $ 23.11万
• 依托单位: BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-04-01 至 2002-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6385488
• 关键词: baboons; bioengineering /biomedical engineering; bioimaging /biomedical imaging; biomagnetism; biophysics; blood brain barrier; cell morphology; contrast media; dogs; hydrogen ions; image processing; intravenous administration; laboratory rat; magnetic resonance imaging; neuroanatomy; nuclear magnetic resonance spectroscopy; sodium ion; surgery; water

The work proposed will continue to explore and demonstrate the range of applications of the effects of non-diamagnetic agents on the two strongest tissue NMR signals: those of 1H2O and 23Na-aq. The adjective non-diamagnetic is used here to indicate any magnetic behavior (ex. paramagnetic, super-paramagnetic, ferromagnetic, etc.) exhibited in addition to simple diamagnetism. Specific projects in the first general category (1H2O) are proposed. The genesis of some of these ideas derived from early work with 23Na-aq. However, now priority must be given to the former, over analogous studies of the latter. It will be demonstrated that one can make 1H2O MR images of contrast reagent (CR) distribution volumes directly, as well as cytolemmal water permeability coefficient maps, cytoplasmic; volume images, and extracellular volume maps. The latter two represent the editing of quantitative (spin density) 1H2O images for the fundamental compartmentalization of biology, which can be radically altered in pathologies such as edemas and tumors. The advance making these development possible is the new technique of combined relaxography and imaging (CRI) recently introduced by this laboratory. The CRI technique is explained in this proposal, and is totally general and applicable to longitudinal, transverse, or rotating-frame NMR relaxation. It can be used to study any CR, whether employing the hyperfine or the bulk magnetic susceptibility mechanisms (or both). Longitudinal relaxation and hyperfine CRs are emphasized in this proposal. This program has recently moved to a new institution and a significantly higher level of effort. Thus, in addition to rat experiments, studies of canine (beagle) and primate (baboon) models are proposed. These involve the programmed, stepped IV infusions of CRs approved for humans, and a quite precedented levels. The ability to study the larger animals provides the opportunity to address important issues of the scaling of the pharmacokinetics and attainable image resolution. The types of images itemized above will be examined for any tissue present in any selected field-of-view, including various muscle groups, the liver, a tumor model implanted in rat thigh muscle, the brain, and a tumor model implanted in the rat brain. The latter two require direct intracerebroventricular injection of the CR since it does not cross the blood-brain-barrier in normal tissue and this approach will be applied only to rats and dogs. The tumor models allow assessment of much greater tissue heterogeneity, and the fundamental bases of their study by the increasingly popular dynamic CR-enhanced method, which involves bolus IV injections. This work involves aspects of physics, physical chemistry, biophysics, and physiology and has ramifications in the study of a number of pathological conditions including neurological and cardiovascular disorders.

拟议的工作将继续探索和展示 非抗磁剂对两种磁性的影响 最强的组织NMR信号：1H 2 O和23 Na-aq的那些。形容词 这里使用非抗磁性来表示任何磁性行为（例如， 顺磁性、超顺磁性、铁磁性等）展出 除了简单的抗磁性。 具体项目先概括 （1H2O）。 这些想法的起源 来自早期的23 Na-aq工作。 但是，现在的优先事项必须是 对前者的研究，与对后者的研究类似。 它将被证明，人们可以使1H 2 O磁共振图像的对比度 试剂（CR）直接分布体积，以及细胞膜水 渗透系数图，细胞质;体积图像，和 细胞外容积图。 后两个代表编辑 定量（自旋密度）1H 2 O图像的基本 生物学的划分，这可以从根本上改变， 病理学如水肿和肿瘤。 这些进展 可能的发展是联合弛豫造影术的新技术， 影像学（CRI）是该实验室最近推出的。 CRI技术 在本建议书中进行了解释，并且完全通用，适用于 纵向、横向或双标架NMR弛豫。 可以 用于研究任何CR，无论是采用超精细还是散装 磁化率机制（或两者）。 纵向弛豫 和超精细CR在这个建议中被强调。这个程序有 最近搬到了一个新的机构， 努力 因此，除了大鼠实验外， （比格犬）和灵长类动物（狒狒）模型。 这些涉及到 程序化的、逐步IV输注的CRs被批准用于人类， 有先例的水平。 研究大型动物的能力 有机会解决扩大联合国系统的重要问题， 药代动力学和可达到的图像分辨率。 的图像的类型 将检查上述项目中存在的任何组织， 视野，包括各种肌肉群，肝脏，肿瘤模型 植入大鼠大腿肌肉、大脑，并植入肿瘤模型 在老鼠的大脑里。 后两者需要直接脑室内 注射CR，因为它不会穿过血脑屏障， 正常组织，该方法将仅应用于大鼠和狗。 肿瘤模型允许评估更大的组织异质性， 以及他们研究的基本基础， 动态CR增强方法，包括静脉推注。 这 工作涉及物理学、物理化学、生物物理学等方面， 生理学，并在一些研究中产生了影响， 病理状况，包括神经和心血管疾病 紊乱

项目成果

Magnetic susceptibility shift selected imaging: MESSI.

磁化率位移选择成像：MESSI。

• DOI: 10.1002/mrm.1910160109
• 发表时间: 1990
• 期刊: Magnetic resonance in medicine
• 影响因子: 3.3
• 作者: Xu,Y;Balschi,JA;SpringerJr,CS
• 通讯作者: SpringerJr,CS

Intimate combination of low- and high-resolution image data: I. Real-space PET and (1)H(2)O MRI, PETAMRI.

低分辨率和高分辨率图像数据的紧密结合：I. 实空间 PET 和 (1)H(2)O MRI、PETAMRI。

• DOI: 10.1002/(sici)1522-2594(199908)42:2<345::aid-mrm17>3.0.co;2-e
• 发表时间: 1999
• 期刊: Magnetic resonance in medicine
• 影响因子: 3.3
• 作者: Sammi,MK;Felder,CA;Fowler,JS;Lee,JH;Levy,AV;Li,X;Logan,J;Pályka,I;Rooney,WD;Volkow,ND;Wang,GJ;SpringerJr,CS
• 通讯作者: SpringerJr,CS

Measurement of metal cation compartmentalization in tissue by high-resolution metal cation NMR.

通过高分辨率金属阳离子 NMR 测量组织中的金属阳离子区室化。

• DOI: 10.1146/annurev.bb.16.060187.002111
• 发表时间: 1987
• 期刊: Annual review of biophysics and biophysical chemistry
• 作者: SpringerJr,CS

Transmembrane ion pumping: high resolution cation NMR spectroscopy.

跨膜离子泵送：高分辨率阳离子核磁共振波谱。

• DOI: 10.1111/j.1749-6632.1987.tb32900.x
• 发表时间: 1987
• 期刊: Annals of the New York Academy of Sciences
• 影响因子: 5.2
• 作者: SpringerJr,CS