MASS SPECTRAL SEQUENCING OF CARBOHYDRATES

碳水化合物的质谱测序

• 批准号: 6285067
• 负责人: VERNON Nye REINHOLD
• 金额: $ 30.97万
• 依托单位: UNIVERSITY OF NEW HAMPSHIRE
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-01-01 至 2004-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6285067
• 关键词: Caenorhabditis elegans; biotechnology; carbohydrate sequence; chemical structure function; electrospray ionization mass spectrometry; functional /structural genomics; glycoprotein structure; glycosylation; mass spectrometry; oligosaccharides; protein structure function; technology /technique development

DESCRIPTION: (Adapted from applicant's abstract) The long range goal of this proposed effort is to understand the structure-functional factors related to molecular glycosylation. The specific aims are directed at analytical problems in two critical areas: methods that will enhance sample capture and detection from biological extracts, and strategies that advance analyte characterization with improved ion trapping (MSn) instrumentation. To consider the most frustrating problem in glycoprotein function, e.g., pleiotrophism, we discuss an innovative genetic approach that begins by building a proteome-glycome data base with the simple animal model, Caenorhabditis elegans. This latter effort is supported by two user groups that will provide glycosyltransferase gene mutants and knockouts to correlate visual and chemical phenotypes, and an 'in-house' geneticist for consultation. To insure an effective focus within the framework of these developments, we additionally summarize continuing applications with two user groups; infectious diseases and glycoconjugate antigen presentation. These "Focus Groups" bring the breath of analytical challenges from isolation and purification to structural characterization. The group activities represent no direct fiscal burden to this application and allow us to contribute to very important issues in immunology. Thus, the "Specific Aims" of the proposal are organized under the heading "Chemical Modifications," (for enhanced isolation and detection), and "Extending Instrumental Capabilities," (by modifications to an ion trap mass spectrometer). A third aim to understand function proposes a combined genetic and structural approach, "Defining Function in Glycobiology," by building a proteome-glycome data base using the first animal model with a defined gene sequence and fully understood cell lineage. The staff supporting for these activities bring considerable experience in each of their representative disciplines discussed in this application; instrumentation, carbohydrate chemistry, proteomics, and glycomics. Additionally, for direct consultation and backup support the University of New Hampshire has an excellent electronics and machine shop in an adjacent building, supported by NASA, a C. elegans geneticist, (Dr. John Collins), who provides worm cultures, incubator time, and genetic understanding, and importantly, a group of chemistry graduate students who seem to possess an appetite for instrumentation and biology whose training is supported by the Department.

描述：（改编自申请人的摘要）这一长期目标 建议的努力是了解结构功能因素相关的 分子糖基化具体目标是针对分析问题 在两个关键领域：加强样本采集和检测的方法 从生物提取物，和战略，推进分析物表征 改进的离子捕获（MSn）仪器。考虑到最 糖蛋白功能中令人沮丧的问题，例如，多效性，我们讨论 一种创新的遗传学方法，首先建立蛋白质组-糖组数据， 以秀丽隐杆线虫为实验动物模型。后一种努力 由两个用户组支持，将提供糖基转移酶基因 突变体和敲除，以关联视觉和化学表型，以及 “内部”遗传学家进行咨询。 为了确保在这些发展的框架内有效地集中注意力，我们 另外总结两个用户组的持续应用程序;传染性 疾病和糖缀合物抗原呈递。这些“焦点小组”带来了 从分离和纯化到 结构表征本集团的活动并不直接涉及财政 负担，使我们能够为非常重要的问题做出贡献 在免疫学上。 因此，提案的“具体目标”是在标题下组织的。 “化学修饰”（用于增强分离和检测），以及 “扩展仪器能力”（通过修改离子阱质量 光谱仪）。了解功能的第三个目的是提出一种组合的遗传学方法， 和结构方法，“糖生物学中的定义功能”，通过建立一个 使用具有确定基因的第一动物模型的蛋白质组-糖组数据库 序列和完全理解的细胞谱系。 支持这些活动的工作人员在每一个方面都有丰富的经验。 本申请中讨论的代表性学科; 仪器、碳水化合物化学、蛋白质组学和糖组学。 此外，为了提供直接咨询和后备支持， 汉普郡有一个很好的电子和机械商店在相邻的 由美国宇航局支持的一个C.优雅的遗传学家，（博士约翰柯林斯），谁 提供蠕虫培养物、孵化器时间和遗传理解， 重要的是，一群化学研究生似乎拥有一个 对仪器和生物学的兴趣，其培训得到了 部门