ANALYSIS OF GENES REGULATING SENSORY NEUROGENESIS

调节感觉神经发生的基因分析

• 批准号: 6322133
• 负责人: Frances Lefcort
• 金额: $ 7万
• 依托单位: MONTANA STATE UNIVERSITY - BOZEMAN
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-03-14 至 2003-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6322133
• 关键词: cell age; cell cycle; cell differentiation; chick embryo; chickens; electroporation; epidermal growth factor; gel electrophoresis; gene expression; gene targeting; genetic regulation; immunocytochemistry; mass spectrometry; mature animal; method development; microinjections; molecular biology; nerve growth factors; neurogenesis; peripheral nervous system; protein structure function; proteomics; spinal ganglion; tissue /cell culture

DESCRIPTION: (Adapted from the applicant's Application) A central question in developmental biology is how a multicellular organism can arise from a single cell. In an effort to understand the complexity of the restricted expression of specific subsets of genes to a particular cell or tissue type, the investigators have compared the transcriptomes (the set of expressed genes) and propose to initiate preliminary studies to determine the feasibility of comparing the proteomes (the set of proteins) of immature and mature chicken dorsal root ganglia (DRG). The objective is to determine the function of a differentially expressed molecule, neural epidermal growth-factor like (NEL), and to extend the analysis of molecular differences to the proteome level. Specific Aim 1 proposes to determine the function of NEL during sensory neurogenesis. Specific Aim 2 plans to determine the molecular differences in the proteomes between immature and mature DRG. A proteomic analysis will be initiated by running 2D gels on E4.5 and E8.5 DRG and, based on their resolution, the feasibility of this approach will be determined. If successful, the investigators can conduct protein identification through mass spectrometric analysis.

描述：（改编自申请人的申请）一个中心问题 是多细胞生物如何从一个 单细胞 为了理解受限的复杂性， 特定细胞或组织类型的特定基因子集的表达， 研究人员比较了转录组（表达的 基因），并建议启动初步研究，以确定 比较未成熟和未成熟的蛋白质组（蛋白质组）的可行性 成熟鸡背根神经节（DRG）。 目标是确定 差异表达分子的功能，神经表皮 生长因子样（NEL），并扩展分子差异的分析 到蛋白质组水平。 具体目标1建议确定 感觉神经发生期间的NEL。 具体目标2计划确定 未成熟和成熟DRG之间蛋白质组的分子差异。 一 通过在E4.5和E8.5 DRG上运行2D凝胶，启动蛋白质组学分析 根据他们的决议，这种方法的可行性将是 测定 如果成功，研究人员可以进行蛋白质 通过质谱分析鉴定。