ANALYSIS OF GENES REGULATING SENSORY NEUROGENESIS

调节感觉神经发生的基因分析

• 批准号: 6530560
• 负责人: Frances Lefcort
• 金额: $ 7万
• 依托单位: MONTANA STATE UNIVERSITY - BOZEMAN
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-03-14 至 2003-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6530560
• 关键词: cell age; cell cycle; cell differentiation; chick embryo; chickens; electroporation; epidermal growth factor; gel electrophoresis; gene expression; gene targeting; genetic regulation; immunocytochemistry; mass spectrometry; mature animal; method development; microinjections; molecular biology; nerve growth factors; neurogenesis; peripheral nervous system; protein structure function; proteomics; spinal ganglion; tissue /cell culture

DESCRIPTION: (Adapted from the applicant's Application) A central question in developmental biology is how a multicellular organism can arise from a single cell. In an effort to understand the complexity of the restricted expression of specific subsets of genes to a particular cell or tissue type, the investigators have compared the transcriptomes (the set of expressed genes) and propose to initiate preliminary studies to determine the feasibility of comparing the proteomes (the set of proteins) of immature and mature chicken dorsal root ganglia (DRG). The objective is to determine the function of a differentially expressed molecule, neural epidermal growth-factor like (NEL), and to extend the analysis of molecular differences to the proteome level. Specific Aim 1 proposes to determine the function of NEL during sensory neurogenesis. Specific Aim 2 plans to determine the molecular differences in the proteomes between immature and mature DRG. A proteomic analysis will be initiated by running 2D gels on E4.5 and E8.5 DRG and, based on their resolution, the feasibility of this approach will be determined. If successful, the investigators can conduct protein identification through mass spectrometric analysis.

描述：（从申请人的申请中改编）一个中心问题 在发育生物学中，多细胞生物如何源于 单细胞。 为了了解受限制的复杂性 基因的特定亚群表达在特定细胞或组织类型上 研究人员比较了转录组（表达的集合 基因）并提议启动初步研究以确定 比较未成熟蛋白质组（蛋白质集）的可行性 成熟的鸡背根神经节（DRG）。 目的是确定 差异表达的分子神经表皮的功能 生长因子（NEL），并扩展分子差异的分析 到蛋白质组水平。 具体目标1提议确定 NEL在感觉神经发生过程中。 具体目标2确定 未成熟和成熟DRG之间蛋白质组织的分子差异。 一个 蛋白质组学分析将通过在E4.5和E8.5 DRG上运行2D凝胶来启动 而且，根据他们的解决方案，这种方法的可行性将是 决定。 如果成功，研究人员可以进行蛋白质 通过质谱分析识别。