ATM AND CELL DEATH IN THE NERVOUS SYSTEM

神经系统中的 ATM 和细胞死亡

• 批准号: 6394017
• 负责人: PETER J MCKINNON
• 金额: $ 21.65万
• 依托单位: ST. JUDE CHILDREN'S RESEARCH HOSPITAL
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-08-01 至 2002-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6394017
• 关键词: BCL2 gene /protein; animal genetic material tag; apoptosis; ataxia telangiectasia; cerebral ischemia /hypoxia; developmental genetics; developmental neurobiology; disease /disorder etiology; embryo /fetus; embryo /fetus toxicology; gene induction /repression; genetically modified animals; ionizing radiation; kainate; laboratory mouse; neurogenetics; neurons; neurotoxicology; nitrous oxide; oxidative stress; p53 gene /protein; tissue /cell culture; tumor suppressor proteins

DESCRIPTION: This is a new proposal, the intention is to investigate At, regulated apoptosis with the ultimate goal of elucidating downstream effectors of Atm signaling. The proposal is based on preliminary data indicating that specific populations of dividing cells in the Atm knockout nervous system are protected from the pro-apoptotic effects of ionizing radiation. The aims of the application will be to identify potential physiologically relevant activators (oxidative stress, excitotoxicity, axotomy) of the Atm signaling pathway by using Atm -/- mice and neuronal cell cultures derived from these animals; to identify components of the apoptotic pathway involving Atm and the nervous system by using the Atm -/- mice as well as p53, Bcl-2 and Bax -/- mice, and cultures derived from these animals and finally, to exhaustively investigate and characterize the developing nervous system in the Atm -/- mice and to ask whether Atm is important for normal programmed cell death.

描述：这是一个新建议，目的是调查 调节凋亡的最终目标是阐明下游 ATM信号的效应子。该提案基于初步数据 表明ATM基因敲除中的特定划分细胞的特定种群 神经系统受到电离的促凋亡影响 辐射。应用程序的目的是确定潜力 生理相关的激活剂（氧化应激，兴奋性毒性， 使用ATM - / - 小鼠和神经元的ATM信号通路的轴切开术） 这些动物衍生出的细胞培养；确定组件 通过使用ATM，涉及ATM和神经系统的凋亡途径 - / - 小鼠以及p53，bcl-2和bax - / - 鼠标，以及源自培养物的培养物 这些动物，最后是详尽的调查和表征 ATM - / - 鼠标中的发育神经系统，询问是否atm是否 对于正常的程序性细胞死亡很重要。