MADLI Mass Spectrometry for Microfluidic Chip Detection

描述（改编自申请人的摘要）：微流体芯片具有 生物分子分析的许多优点如低成本，小尺寸， 低样品消耗、高灵敏度和快速分析， 可用的检测方法有限。 这个研究项目的目的 是开发一种新的微流控芯片检测器， 使用用于在线MALDI的旋转球入口（ROBIN）接口进行光谱分析。 旋转球充当机械输送机构， 将微流控芯片上的化合物输送到飞行时间质谱仪， 激光解吸和电离。 将基质溶液与 在到达旋转球界面之前， 沉积并干燥以形成MALDI靶。 ROBIN MALDI接口是一个 一种新的在线MALDI液相引入方法及其初步研究 表明其可行性已得到实施。 接口兼容 与任何现有的MALDI矩阵，并不遭受记忆效应。 的 本提案中描述的研究涉及一种合作， 在格鲁吉亚理工学院的微流体设备设计和制造方面的专业知识， 埃默里大学在MALDI质谱方面的专业知识 这些团体将共同 开发芯片到球接口和质谱仪连接， 允许MALDI用作微流控芯片检测器。 微流体芯片 ROBIN MALDI将对高通量生物分子产生重大影响 蛋白质组学和基因组学应用的分析。 在潜在的应用中， 蛋白质可以在芯片上纯化、分离、消化， 分析了 DNA可以从单个细胞中提取，通过PCR扩增， 测序、基因探针或其它分析。 质谱法通常是 它比荧光检测提供更多的信息， 分子量结构信息来自碎片模式。 MALDI是 比电喷雾电离更能容忍杂质， 当启动和停止时，信号不稳定，电喷雾也是如此。 小说 芯片制造程序将被开发用于构建一个强大的 芯片和质谱仪之间的接口。 这些发展将 在微流控芯片互连和接口中具有广泛的适用性。

DESCRIPTION (Adapted from the applicant's abstract): Microfluidic chips have many advantages for the analysis of biomolecules such as low cost, small size, low sample consumption, high sensitivity, and rapid analysis, yet the available methods of detection are limited. The goal of this research project is to develop a new detector for microfluidic chips using MALDI mass spectrometry with a rotating ball inlet (ROBIN) interface for on-line MALDI. The rotating ball acts as mechanical transport mechanism that delivers the compounds on the microfluidic chip to a time-of-flight mass spectrometer for laser desorption and ionization. The matrix solution is mixed with the analyte on the chip before reaching the rotating ball interface where it is deposited and dried to form the MALDI target. The ROBIN MALDI interface is a novel approach to on-line MALDI liquid introduction and preliminary studies indicating its feasibility have been performed. The interface is compatible with any existing MALDI matrix and does not suffer from memory effects. The research described in this proposal involves a collaboration that combines the expertise at Georgia Tech in microfluidic device design and fabrication with the expertise at Emory in MALDI mass spectrometry. The groups will jointly develop the chip to ball interface and mass spectrometer connection that will allow MALDI to be used as a microfluidic chip detector. Microfluidic chip ROBIN MALDI will have a significant impact on high throughput biomolecule analysis for proteomics and genomics applications. In potential applications, proteins can be purified, separated, digested on-chip and then rapidly mass analyzed. DNA can be extracted from individual cells, amplified by PCR for sequencing, gene probe, or other analysis. Mass spectrometry is generally applicable and provides more information than fluorescence detection such as molecular weight structural information from fragmentation patterns. MALDI is more tolerant of impurities than electrospray ionization and does not suffer from signal instability when started and stopped as does electrospray. Novel chip fabrication procedures will be developed for the construction of a robust interface between the chip and the mass spectrometer. These developments will have broad applicability in microfluidic chip interconnection and interfacing.