MADLI Mass Spectrometry for Microfluidic Chip Detection

用于微流控芯片检测的 MADLI 质谱法

• 批准号: 6540646
• 负责人: Kermit King Murray
• 金额: $ 8.96万
• 依托单位: LOUISIANA STATE UNIV A&M COL BATON ROUGE
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-08-01 至 2004-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6540646
• 关键词: bioassay; mass spectrometry; matrix assisted laser desorption ionization; microprocessor /microchip; technology /technique development

DESCRIPTION (Adapted from the applicant's abstract): Microfluidic chips have many advantages for the analysis of biomolecules such as low cost, small size, low sample consumption, high sensitivity, and rapid analysis, yet the available methods of detection are limited. The goal of this research project is to develop a new detector for microfluidic chips using MALDI mass spectrometry with a rotating ball inlet (ROBIN) interface for on-line MALDI. The rotating ball acts as mechanical transport mechanism that delivers the compounds on the microfluidic chip to a time-of-flight mass spectrometer for laser desorption and ionization. The matrix solution is mixed with the analyte on the chip before reaching the rotating ball interface where it is deposited and dried to form the MALDI target. The ROBIN MALDI interface is a novel approach to on-line MALDI liquid introduction and preliminary studies indicating its feasibility have been performed. The interface is compatible with any existing MALDI matrix and does not suffer from memory effects. The research described in this proposal involves a collaboration that combines the expertise at Georgia Tech in microfluidic device design and fabrication with the expertise at Emory in MALDI mass spectrometry. The groups will jointly develop the chip to ball interface and mass spectrometer connection that will allow MALDI to be used as a microfluidic chip detector. Microfluidic chip ROBIN MALDI will have a significant impact on high throughput biomolecule analysis for proteomics and genomics applications. In potential applications, proteins can be purified, separated, digested on-chip and then rapidly mass analyzed. DNA can be extracted from individual cells, amplified by PCR for sequencing, gene probe, or other analysis. Mass spectrometry is generally applicable and provides more information than fluorescence detection such as molecular weight structural information from fragmentation patterns. MALDI is more tolerant of impurities than electrospray ionization and does not suffer from signal instability when started and stopped as does electrospray. Novel chip fabrication procedures will be developed for the construction of a robust interface between the chip and the mass spectrometer. These developments will have broad applicability in microfluidic chip interconnection and interfacing.

描述（改编自申请人的摘要）：微流体芯片具有 分析生物分子的许多优势，例如低成本，小尺寸， 样本消耗少，灵敏度高和快速分析，但是 可用的检测方法有限。 该研究项目的目标 是使用MALDI质量开发用于微流体芯片的新检测器 具有旋转球入口（Robin）界面的光谱法，用于在线MALDI。 旋转球充当机械运输机构 微流体芯片上的化合物到飞机质谱仪的 激光解吸和电离。 矩阵溶液与 在到达旋转球界面之前，分析物在芯片上 沉积并干燥以形成MALDI目标。 Robin Maldi界面是 新的在线MALDI液体引入和初步研究的方法 表明其可行性已执行。 该界面兼容 对于任何现有的MALDI矩阵，都不会遭受记忆效应。 这 该提案中描述的研究涉及一项合作，将 佐治亚理工学院在微流体设备设计和制造方面的专业知识 MALDI质谱法的Emory的专业知识。 小组将共同 将芯片与球接口和质谱仪连接开发 允许MALDI用作微流体芯片检测器。 微流体芯片 罗宾·马尔迪（Robin Maldi）将对高通量生物分子产生重大影响 分析蛋白质组学和基因组学应用。 在潜在应用中， 蛋白质可以在片上纯化，分离，消化，然后快速质量 分析。 DNA可以从单个细胞中提取，通过PCR扩增 测序，基因探针或其他分析。 质谱法通常是 适用并提供更多的信息，而不是荧光检测 分子量的结构信息来自碎片模式。 马尔迪是 比电喷雾电离更宽容杂质，不会遭受痛苦 从启动时信号不稳定性和电喷雾停止。 小说 将开发芯片制造程序，以建造可靠的 芯片和质谱仪之间的接口。 这些事态发展会 在微流体芯片互连和接口中具有广泛的适用性。