REAL TIME MASS SPECTROMETRY OF BIOAEROSOLS

生物气溶胶的实时质谱分析

• 批准号: 6489871
• 负责人: Kermit King Murray
• 金额: $ 14.7万
• 依托单位: LOUISIANA STATE UNIV A&M COL BATON ROUGE
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-01-08 至 2003-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6489871
• 关键词: aerosols; bacteria; matrix assisted laser desorption ionization; method development; microorganism culture; portable biomedical equipment; spores; virus

DESCRIPTION: (Adapted from applicant's abstract) The goal of this research program is to develop a mass spectrometry technique for the determination of bioaerosol concentration in air. The work is divided into three parts. First, an off-line method for microbe and bioaerosol ionization will be developed which uses a liquid matrix for matrix-assisted laser desorption ionization (MALDI). Second, the liquid matrix method will be used with an on-line aerosol mass spectrometer that detects single particles in a known air volume and tallies the number of bioaerosol particles. Third, bioaerosols will be collected and cultured to determine the number of colony forming units (CFU) in a given volume. The results will be compared to those obtained with the aerosol mass spectrometer.

描述：(摘自申请者的摘要)本研究的目的 计划是开发一种质谱学技术来测定 空气中的生物气溶胶浓度。这项工作分为三个部分。第一, 将开发一种用于微生物和生物气溶胶电离的离线方法 它使用液体基质进行基质辅助激光解吸电离 (MALDI)。第二，液体基质方法将与在线气溶胶一起使用 质谱计，检测已知风量中的单个粒子和 与生物气溶胶颗粒的数量相吻合。第三，生物气雾剂将 收集并培养，测定细胞集落形成单位(CFU)的数量 给定的音量。结果将与使用气雾剂获得的结果进行比较。 质谱仪。