NEUROSCIENCE

神经科学

• 批准号: 6505138
• 负责人: JOSEPH A WHITTAKER
• 金额: $ 20.08万
• 依托单位: MOREHOUSE SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-01 至 2002-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6505138
• 关键词: basal ganglia; brain electrical activity; chordate locomotion; circadian rhythms; ethology; laboratory rat; suprachiasmatic nucleus

There is evidence to suggest that the release of dopamine within the basal ganglia exhibits a circadian pattern and suggests a similar pattern in basal ganglia function. Immunocytochemical approaches will be used to study the distribution of dopamine and EAA receptors in the rat substantia nigra. The effects of circadian rhythms on these receptor distributions will be determined in melatonin-treated animals, as well as in others entrained to various lighting conditions. Subsequently, electrophysiological determinations of DA function, relative to motor mechanisms in the basal ganglia, will be evaluated. In addition, the humoral effects of melatonin on DA and EAA modulatory mechanisms in the substantia nigra will be studied in brain slices using conventional current-clamp as well as whole cell patch clamp techniques. We will also identify the functional role of each receptor subtype utilizing electrophysiological and pharmacological approaches (Aim II). Attempts will be made to correlate functional parameters with light/dark cycles and melatonin levels. Our findings will (i) contribute to the further understanding of glutamate and DA functions in the brain (ii) elucidate the identity and possible contribution of select DA and glutamate receptors in mechanisms of normal SNc function and (iii) delineate their role in circadian or melatonin control of CNS DA neuronal function.

有证据表明，多巴胺的释放在基底 神经节表现出昼夜节律模式，并表明在 基底神经节功能 免疫细胞化学方法将用于 研究多巴胺和EAA受体在大鼠脑实质的分布 黑鬼 昼夜节律对这些受体分布的影响 将在褪黑激素处理的动物中确定，以及在其他动物中， 在各种照明条件下使用。 随后，委员会注意到， DA功能的电生理学测定，相对于运动 将评估基底神经节中的机制。 此外该 褪黑素对多巴胺和兴奋性氨基酸调节机制的体液作用 黑质将在脑切片中使用常规的 电流钳以及全细胞膜片钳技术。 我们还将 确定每种受体亚型的功能作用， 电生理学和药理学方法（目的II）。 尝试 将使功能参数与光/暗周期相关， 褪黑激素水平 我们的研究结果将（i）有助于进一步 了解谷氨酸和DA在大脑中的功能（ii）阐明 选择DA和谷氨酸的鉴别和可能的贡献 受体在正常SNc功能的机制和（iii）描绘他们的 在CNS DA神经元功能的昼夜节律或褪黑素控制中的作用。