ACQUISITION OF A 400 MHZ NMR SPECTROMETER

这项提案要求为一台400 MHz宽带核磁共振光谱仪提供资金，该光谱仪将作为多用户、跨学科资源发挥作用。该仪器将为需要高场核磁共振的研究人员提供最先进的能力，以表征通过化学和生化合成产生的中小分子的结构。五个主要用户都得到了NIH的支持，将受益于该仪器以及几个次要用户，尽管该仪器将提供给阿肯色大学医学科学大学的整个研究社区。主要研究领域包括酶底物和抑制剂的设计和合成，生物过程代谢物的鉴定，以及药物处理对动物细胞和组织悬浮液的影响的表征。适用于常规L-D和复杂的2-D实验的“步行”核磁共振设备的可获得性将极大地促进UAMS的生物医学研究。

This Proposal requests funding for a 400 MHz broadband NMR spectrometer that will function as a multi-user, interdisciplinary resource. The instrument will provide state-of-the-art capabilities for researchers requiring high field NMR for structural characterization of small to medium-sized molecules produced through chemical and biochemical synthesis. Five major users, all with NIH support, will benefit from the instrument as well as several minor users, although the instrument will be available to the entire research community at the University of Arkansas for Medical Sciences. Major areas of research include design and synthesis of enzyme substrates and inhibitors, identification of metabolites of biological processes, and characterization of effects of drug treatments on animal cell and tissue suspensions. The availability of a "walk up" NMR facility that is suitable for routine l-D as well as sophisticated 2-D experiments will greatly facilitate biomedical research at UAMS.