ACQUISITION OF A 400 MHZ NMR SPECTROMETER

该提案要求为400 MHz宽带NMR光谱仪提供资金，该光谱仪将作为多用户、跨学科资源。该仪器将为需要高场NMR的研究人员提供最先进的能力，以表征通过化学和生物化学合成产生的中小分子的结构。五个主要用户，都与NIH的支持，将受益于仪器以及几个小用户，虽然该仪器将提供给整个研究界在阿肯色州医学科学。主要研究领域包括酶底物和抑制剂的设计和合成，生物过程代谢物的鉴定，以及药物治疗对动物细胞和组织悬浮液的影响的表征。一个“走”NMR设备，适合于常规的一维以及复杂的二维实验的可用性将大大促进在UAMS的生物医学研究。

This Proposal requests funding for a 400 MHz broadband NMR spectrometer that will function as a multi-user, interdisciplinary resource. The instrument will provide state-of-the-art capabilities for researchers requiring high field NMR for structural characterization of small to medium-sized molecules produced through chemical and biochemical synthesis. Five major users, all with NIH support, will benefit from the instrument as well as several minor users, although the instrument will be available to the entire research community at the University of Arkansas for Medical Sciences. Major areas of research include design and synthesis of enzyme substrates and inhibitors, identification of metabolites of biological processes, and characterization of effects of drug treatments on animal cell and tissue suspensions. The availability of a "walk up" NMR facility that is suitable for routine l-D as well as sophisticated 2-D experiments will greatly facilitate biomedical research at UAMS.