NONLINEAR OPTICAL CONFOCAL MICROSCOPE

我们申请资金购买蔡司LSM 510 NLO共聚焦显微镜，并配备用于多光子显微镜的相干MiraX-ZFC激光系统。七个主要用户和其他11个用户，由美国国立卫生研究院（26个独立的研究赠款）资助，描述了20个研究项目，涵盖了广泛的生物学问题，并将共同利用仪器的所有功能。申请人代表医学院的七个不同部门。院长已作出承诺的$200，000的设施的支持，和其他教师作出了额外的资金，设备和空间的承诺。Winfried Denk博士（贝尔实验室）将担任外部顾问。他和Graham Ellis-Davies博士（费城）将与主要用户合作开展两个研究项目。一个新的毕业生6course，“活细胞中细胞功能的光学测量”将作为扩大光学显微镜设施的一部分提供。主要用户在各种成像技术方面有着丰富的经验，并已获得合理的初步数据，这些数据说明了对该设备的需求和拟议实验的可行性。对现有设备的全面清点和对提案的仔细审查清楚地表明，在健康科学中心或合理附近的任何其他校园，没有一个项目是用现有设备完成的。在过去十年中出现的由相同人员持续管理共享光学设施的稳定和经过时间考验的管理计划将确保仪器得到良好的维护，使其能够自我支持，并由来自用户组和随着时间的推移来自该地区更多科学家的训练有素的科学家集中使用。

We request funds to purchase a Zeiss LSM 510 NLO confocal microscope, augmented by a Coherent MiraX-ZFC laser system for multi- photon microscopy. Seven Major Users and eleven other users, well funded by NIH (26 separate research grants), describe twenty research projects that span a broad range of biological questions and will collectively utilize all of the capabilities of the instrument. The applicants represent seven different departments in the School of Medicine. The Dean has made a commitment of $200,000 for support of the facility, and other faculty have made additional commitments of funds, equipment, and space. Dr. Winfried Denk (Bell Labs) will serve as External Advisor. He and Dr. Graham Ellis-Davies (Philadelphia) will collaborate with Major Users on two research projects. A new graduate 6ourse, "Optical Measurement of Cellular Function in Live Cells" will be offered as part of an expanding optical microscopy facility. Major Users have had extensive experience with various imaging techniques, and have acquired sound preliminary data that illustrate both the need for this equipment and the feasibility of the proposed experiments. A comprehensive inventory of existing equipment and careful scrutiny of proposals make clear that none of the projects can be accomplished with current equipment at the Health Science Center or any other campus in reasonable proximity. A stable and time-tested management plan that has emerged over the past ten years of continuous administration of shared optical facilities by the same personnel will insure that the instrument will be well maintained, that it will become self-supporting, and that it will be used intensively by well- trained scientists drawn both from the User Group and, over time, from the larger population of scientists in the region.