NONLINEAR OPTICAL CONFOCAL MICROSCOPE

我们申请资金购买一个蔡司LSM 510 NLO共聚焦显微镜，增加一个相干MiraX-ZFC激光系统用于多光子显微镜。7个主要用户和11个其他用户，由NIH资助（26个单独的研究资助），描述了20个研究项目，涵盖了广泛的生物学问题，并将共同利用该仪器的所有功能。申请人代表了医学院七个不同的系。院长承诺为该设施提供20万美元的支持，其他教师也承诺提供资金、设备和空间。Winfried Denk博士（贝尔实验室）将担任外部顾问。他和Graham Ellis-Davies博士（费城）将在两个研究项目上与Major Users合作。一门新的研究生课程“活细胞细胞功能的光学测量”将作为扩大的光学显微镜设施的一部分提供。主要用户在各种成像技术方面具有丰富的经验，并获得了良好的初步数据，说明对这种设备的需要和拟议实验的可行性。对现有设备的全面盘点和对提案的仔细审查清楚地表明，没有一个项目可以用健康科学中心或任何其他合理邻近校园的现有设备来完成。在过去的十年中，由相同的人员持续管理共享光学设施，形成了一个稳定的、经过时间考验的管理计划，这将确保仪器得到良好的维护，它将成为自给自足的，并且它将被来自用户组和随着时间的推移，来自该地区更大的科学家人口的训练有素的科学家集中使用。

We request funds to purchase a Zeiss LSM 510 NLO confocal microscope, augmented by a Coherent MiraX-ZFC laser system for multi- photon microscopy. Seven Major Users and eleven other users, well funded by NIH (26 separate research grants), describe twenty research projects that span a broad range of biological questions and will collectively utilize all of the capabilities of the instrument. The applicants represent seven different departments in the School of Medicine. The Dean has made a commitment of $200,000 for support of the facility, and other faculty have made additional commitments of funds, equipment, and space. Dr. Winfried Denk (Bell Labs) will serve as External Advisor. He and Dr. Graham Ellis-Davies (Philadelphia) will collaborate with Major Users on two research projects. A new graduate 6ourse, "Optical Measurement of Cellular Function in Live Cells" will be offered as part of an expanding optical microscopy facility. Major Users have had extensive experience with various imaging techniques, and have acquired sound preliminary data that illustrate both the need for this equipment and the feasibility of the proposed experiments. A comprehensive inventory of existing equipment and careful scrutiny of proposals make clear that none of the projects can be accomplished with current equipment at the Health Science Center or any other campus in reasonable proximity. A stable and time-tested management plan that has emerged over the past ten years of continuous administration of shared optical facilities by the same personnel will insure that the instrument will be well maintained, that it will become self-supporting, and that it will be used intensively by well- trained scientists drawn both from the User Group and, over time, from the larger population of scientists in the region.