NONLINEAR OPTICAL CONFOCAL MICROSCOPE

我们请求资金购买蔡司LSM 510NLO共焦显微镜，并配备用于多光子显微镜的相干Mirax-ZFC激光系统。7个主要用户和11个其他用户，由NIH(26个单独的研究拨款)提供充足的资金，描述了20个研究项目，这些项目涵盖了广泛的生物学问题，并将共同利用该仪器的所有能力。申请者来自医学院的七个不同部门。院长已经承诺为该设施提供20万美元的支持，其他教职员工也做出了额外的资金、设备和空间承诺。温弗里德·登克博士(贝尔实验室)将担任外部顾问。他和Graham Ellis-Davies博士(费城)将在两个研究项目上与主要用户合作。一门新的研究生课程“活细胞中细胞功能的光学测量”将作为扩展的光学显微镜设施的一部分提供。主要用户对各种成像技术有丰富的经验，并获得了可靠的初步数据，说明了对该设备的需求和拟议实验的可行性。对现有设备的全面清点和对提案的仔细审查清楚地表明，在健康科学中心或距离合理的任何其他校园，利用现有设备无法完成任何项目。在过去十年由同一人员持续管理共享光学设施的过程中出现了一个稳定且经过时间考验的管理计划，该计划将确保仪器得到很好的维护，使其成为自给自足的仪器，并确保该仪器将由训练有素的科学家密集使用，这些科学家既来自用户组，也随着时间的推移，从该地区更多的科学家中挑选出来。

We request funds to purchase a Zeiss LSM 510 NLO confocal microscope, augmented by a Coherent MiraX-ZFC laser system for multi- photon microscopy. Seven Major Users and eleven other users, well funded by NIH (26 separate research grants), describe twenty research projects that span a broad range of biological questions and will collectively utilize all of the capabilities of the instrument. The applicants represent seven different departments in the School of Medicine. The Dean has made a commitment of $200,000 for support of the facility, and other faculty have made additional commitments of funds, equipment, and space. Dr. Winfried Denk (Bell Labs) will serve as External Advisor. He and Dr. Graham Ellis-Davies (Philadelphia) will collaborate with Major Users on two research projects. A new graduate 6ourse, "Optical Measurement of Cellular Function in Live Cells" will be offered as part of an expanding optical microscopy facility. Major Users have had extensive experience with various imaging techniques, and have acquired sound preliminary data that illustrate both the need for this equipment and the feasibility of the proposed experiments. A comprehensive inventory of existing equipment and careful scrutiny of proposals make clear that none of the projects can be accomplished with current equipment at the Health Science Center or any other campus in reasonable proximity. A stable and time-tested management plan that has emerged over the past ten years of continuous administration of shared optical facilities by the same personnel will insure that the instrument will be well maintained, that it will become self-supporting, and that it will be used intensively by well- trained scientists drawn both from the User Group and, over time, from the larger population of scientists in the region.