3I Spinning Disk Confocal Microscope system

3I 转盘共焦显微镜系统

• 批准号: 7794436
• 负责人: WILLIAM J BETZ
• 金额: $ 50万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-29 至 2011-04-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7794436
• 关键词: Data; Electrophysiology (science); Equipment; Fluorescence Microscopy; Funding; Grant; Housing; Image; Institution; Lasers; Life; Maintenance; Microscope; Minor; Research Personnel; Research Project Grants; Resolution; Security; System; Thick; Tissues; United States National Institutes of Health; cellular imaging; experience; innovation; instrument; lens; light microscopy

DESCRIPTION (provided by applicant): Spinning Disk Confocal Microscope system. Eleven Major Users and 4 Minor Users, each with ongoing NIH grant support and extensive technical experience and expertise in fluorescence microscopy, request funds to purchase a Marianas SDC spinning disk microscope from Intelligent Imaging Innovations (3I). Two microscopes (one inverted and one upright) will share a Yokogawa CSU-X1 micro-lens spinning disk unit and 4 excitation laser lines (405, 488, 561, and 640 nm). Other accessories are described below. Significant institutional funds ($100,000) will assist in purchase of the instrument, which will be housed in and maintained by the campus-wide Light Microscopy Facility, making it broadly available to researchers and insuring successful long-term maintenance and security. All 15 research projects involve live cell imaging. Extensive pilot data have demonstrated the unique capabilities of the instrument, such as greatly reduced photo-damage and photo-bleaching, rapid acquisition of multiple image stacks, and ability to achieve diffraction-limited resolution (inverted microscope) and accommodate thick tissue imaging and electrophysiology (upright microscope). None of the proposed research projects can be accomplished with any equipment available to us on our campus or at neighboring institutions.

描述（由申请人提供）：旋转圆盘共聚焦显微镜系统。11个主要用户和4个次要用户，每个都有持续的NIH资助支持和丰富的技术经验和荧光显微镜的专业知识，要求资金从智能成像创新（3 I）购买马里亚纳SDC旋转圆盘显微镜。两个显微镜（一个倒置，一个直立）将共享一个Yokogawa CSU-X1微透镜旋转盘单元和4个激发激光线（405，488，561和640 nm）。其他附件如下所述。大量的机构资金（100 000美元）将协助购买该仪器，该仪器将被安置在整个校园的光学显微镜设施中并由其维护，使其广泛提供给研究人员，并确保成功的长期维护和安全。所有15个研究项目都涉及活细胞成像。大量的试验数据已经证明了该仪器的独特功能，例如大大减少了光损伤和光漂白，快速获取多个图像堆栈，以及实现衍射限制分辨率（倒置显微镜）和适应厚组织成像和电生理学（直立显微镜）的能力。没有一个拟议的研究项目可以用我们在校园或邻近机构提供的任何设备来完成。