LC-ELECTROSPRAY QUADRUPOLE ION TRAP MASS SPECTROMETER

LC 电喷雾四极杆离子阱质谱仪

• 批准号: 6051410
• 负责人: DOMINIC M DESIDERIO
• 金额: $ 24.28万
• 依托单位: UNIVERSITY OF TENNESSEE HEALTH SCI CTR
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-02-01 至 2001-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6051410
• 关键词: biomedical equipment purchase; electrospray ionization mass spectrometry

Funds are requested to purchase a liquid chromatograph-electrospray quadrupole ion trap mass spectrometer (LC-ESI-Q-IT MS). The LC-ESI- Q-IT MS is selected because it possesses several critical operating features: l) high detection sensitivity (pmol-fmol), 2) on-line coupling of HPLC to MS, 3) excellent MST capabilities, and 4) high throughput and automation. This instrument will be shared by a group of NIH-supported researchers at the University of Tennessee, Memphis, and at the St. Jude Children's Research Hospital, Memphis. The requested LC-ESI-Q-IT MS is needed to complement the current MALDI-TOF instrument and to provide an alternative to MALDI-TOF analysis. The LC-ESI-Q-IT instrument will be employed in the following research programs: Proteomics: (i) studies of the alterations of opioid neuropeptidergic systems in human cerebrospinal fluid (CSF), that are associated with idiopathic low back pain; (ii) analysis of the opioid systems in human pituitary tumor tissue; and (iii) investigation of the proteases involved in the activation of human macrophages for enhanced killing of microbes and tumor cells. Quantification: (i) neuropeptides in human pituitary and in human CSF; (ii) corticotropin-releasing hormone in the microdialysates of mouse CSF (in the investigation of the effects of nicotine on neuronal functions); (iii) neuropeptide Y and orexins in rat hypothalamus (for the study of the nicotine-induced changes in food intake stimulators); and (iv) synthetic opioid peptide analogs in ovine plasma (in the study of pain-reducing drugs for use in obstetrics). Structures of biologically active compounds: (i) bacterial cell wall constituents (lipopolysaccharides and glycopeptides) involved in macrophage activation; and (ii) blood-derived phospholipid growth factors. Protein characterization: (i) enzymes involved in arachidonic acid release in blood vessels; (ii) wild-type and mutant forms of thiopurine methyltransferase (for the investigation of the genetic polymorphism of TPMT); and (lii) mismatch repair proteins (in the study of their involvement in the determination of thiopurine cytotoxicity). Successful completion of these research programs will further the understanding in several areas related to human health, such as the role of neuropeptides in disease, effects of smoking.on brain functions, ability of the immune system to fight infection, and the use of thiopurines for the treatment of childhood cancer.

需要资金购买一台液相色谱-电喷雾四极杆离子阱质谱仪。选择LC-ESI- Q-IT MS是因为它具有几个关键的操作特征：1）高检测灵敏度（pmol-fmol），2）HPLC与MS的在线耦合，3）优异的MST能力，以及4）高通量和自动化。该仪器将由孟菲斯田纳西大学和孟菲斯圣裘德儿童研究医院的一组NIH支持的研究人员共享。需要所要求的LC-ESI-Q-IT MS来补充当前的MALDI-TOF仪器，并提供MALDI-TOF分析的替代方案。LC-ESI-Q-IT仪器将用于以下研究项目：蛋白质组学：（i）研究与特发性腰痛相关的人脑脊液（CSF）中阿片类神经肽能系统的改变;（ii）分析人垂体瘤组织中的阿片类系统;和（iii）研究参与激活人巨噬细胞以增强对微生物和肿瘤细胞的杀伤的蛋白酶。定量：（i）人垂体和人CSF中的神经肽;（ii）小鼠CSF微透析液中的促肾上腺皮质激素释放激素（研究尼古丁对神经元功能的影响）;（iii）大鼠下丘脑中的神经肽Y和食欲素（用于研究尼古丁引起的食物摄入刺激物的变化）;和（iv）绵羊血浆中的合成阿片肽类似物（在用于产科的止痛药物的研究中）。生物活性化合物的结构：（i）参与巨噬细胞活化的细菌细胞壁成分（脂多糖和糖肽）;和（ii）血液来源的磷脂生长因子。蛋白质表征：（i）参与血管中花生四烯酸释放的酶;（ii）野生型和突变形式的巯基嘌呤甲基转移酶（用于研究TPMT的遗传多态性）;和（lii）错配修复蛋白（用于研究它们参与巯基嘌呤细胞毒性的测定）。这些研究项目的成功完成将进一步了解与人类健康有关的几个领域，如神经肽在疾病中的作用，吸烟对大脑功能的影响，免疫系统对抗感染的能力，以及使用硫嘌呤治疗儿童癌症。