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Q-TOF Hybrid Quadrupole/Orthogonal TOF Mass Spectrometer

Q-TOF 混合四极杆/正交 TOF 质谱仪

基本信息

批准号：
6733128
负责人：
DOMINIC M DESIDERIO
金额：
$ 49.98万
依托单位：
UNIVERSITY OF TENNESSEE HEALTH SCI CTR
依托单位国家：
美国
项目类别：
财政年份：
2004
资助国家：
美国
起止时间：
2004-04-01 至 2006-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6733128
关键词：
biomedical equipment purchase mass spectrometry matrix assisted laser desorption ionization

项目摘要

DESCRIPTION (provided by applicant): This revised shared instrument grant application requests funds to purchase a state-of-the-art matrix-assisted laser desorption/ionization hybrid quadrupole time-of-flight mass spectrometer (MALDI-QTOF MS). The current MALDI-TOF instrument will be decommissioned in 2003. A group of 16 NIH-funded researchers will be the major users of the requested instrumentation, and 12 other users will use the instrument for projects that are not currently NIH-funded. We estimate that a minimum of 250 samples will be analyzed per week, and that MS/MS data will be needed for the majority of those samples. A significant new development in Memphis is the new UTHSC Cancer Institute. The Boston-Baskins Cancer Group has entered into a partnership with UTHSC. A temporary director has been hired, an NIH NCI P21 planning grant has been submitted (March, 2003), a permanent director will be hired, two new buildings will be constructed, and new faculty will be hired. Dr. Desiderio will direct the UT Cancer Institute Proteomics Laboratory. Many more researchers will be hired, and many will need a MALDI QTOF MS for their research programs. Many investigators from various disciplines have been using our rapidly aging MALDI-TOF instrument, and they have extensive experience with the sample preparation methods for MALDI and with the interpretation of MALDI data. Advantages of the requested instrument include the ability to prepare samples before instrument time, the ability to reanalyze samples whenever necessary, and (very important) the user-friendliness of the instrumentation. It is significant to note that our users operate the MALDI-TOF instruments. The users of our aging MALDI-TOF mass spectrometer are acutely aware of its rapidly decreasing performance in terms of sensitivity and mass accuracy, and of its lack of sequencing capabilities. Therefore, we will remove our MALDI -TOF MS from service in 2003. Often, experiments must be repeated because the MALDI-TOF analysis has failed, or because amino acid sequence data are necessary - but cannot be obtained with the MALDI-TOF. Clearly, a state-of-the-art MALDI-based mass spectrometer with MS/MS capabilities is needed to support the rapidly increasing number of extensive and diverse research programs on the UTHSC campus.

描述（由申请人提供）：此修订的共享仪器赠款申请要求资金购买最先进的基质辅助激光解吸/电离混合四极杆飞行时间质谱仪（MALDI-QTOF MS）。目前的MALDI-TOF仪器将于2003年退役。一组由16名NIH资助的研究人员将成为所要求的仪器的主要用户，另外12名用户将使用该仪器进行目前没有NIH资助的项目。我们估计每周至少分析250份样品，其中大部分样品需要MS/MS数据。孟菲斯的一个重要的新发展是新的UTHSC癌症研究所。波士顿-巴斯金斯癌症集团已与UTHSC建立合作伙伴关系。已经聘请了一名临时主任，已经提交了NIH NCI P21规划赠款（2003年3月），将聘请一名永久主任，将建造两座新建筑，并将聘请新的教师。Desiderio博士将领导UT癌症研究所蛋白质组学实验室。更多的研究人员将被雇用，许多人将需要一个MALDI QTOF MS为他们的研究计划。来自不同学科的许多研究人员一直在使用我们快速老化的MALDI-TOF仪器，他们在MALDI样品制备方法和MALDI数据解释方面拥有丰富的经验。所需仪器的优点包括能够在仪器使用前制备样品，能够在必要时重新分析样品，以及（非常重要的）仪器的用户友好性。值得注意的是，我们的用户操作MALDI-TOF仪器。我们老化的MALDI-TOF质谱仪的用户敏锐地意识到其在灵敏度和质量准确性方面的性能迅速下降，并且缺乏测序能力。因此，我们将在2003年停止使用我们的MALDI-TOF MS.通常，实验必须重复，因为MALDI-TOF分析失败，或者因为氨基酸序列数据是必要的，但不能用MALDI-TOF获得。显然，一个国家的最先进的MALDI为基础的质谱仪与MS/MS的能力是必要的，以支持在UTHSC校园广泛和多样化的研究计划的数量迅速增加。