Control of Biofilms by Natural Products

描述(由申请人提供)：生物膜在慢性和慢性疾病中的重要性 治疗耐药的传染病，越来越明显。生物膜也 是工业过程中的一个主要问题。这项提议的重点是 开发发现阻止和/或逆转生物被膜的化合物的方法 形成，并发现和结构表征，活性化合物。 具体地说，我们将开发有效的方法来筛选提取物和 化合物具有调节革兰氏阴性病原体生物被膜的能力， 并开发和验证具有革兰氏阴性细菌生物膜的新筛查 和真菌。此外，我们将评估植物，特别是那些生活在 潮湿或潮湿的环境，可能是新化合物的丰富来源 阻止或逆转生物膜的形成。化合物将被分离出来并 以灵敏、高通量的并行LC-MS和LC-核磁共振为特征。一次 我们定义了丰富的化合物来源，有效的分析，并确定了新的 在第一阶段和第二阶段能够阻止或逆转生物被膜形成的化合物 我们将使用在这里优化的方法，并进一步表征生物 活性化合物的活性，以确定其应用潜力(S) 在防止生物膜相关的损害和疾病方面，以及有效性 我们发现有价值化合物的方法。 建议的商业应用：不可用

DESCRIPTION (provided by applicant): The importance of Biofilms in chronic and treatment-resistant infectious disease, increasingly is clear. Biofilms also are a major problem in industrial processes. The focus of this proposal is to develop methods for discovering compounds that block and/or reverse biofilm formation, and to discover, and structurally characterize, active compounds. Specifically, we will develop efficient methods to screen extracts and compounds for their ability to regulate biofilms of a gram negative pathogen, and develop and validate new screens with biofilms of gram negative bacteria and fungi. In addition, we will evaluate plants, especially those that live in moist or wet environments, as a possible rich source of novel compounds that block or reverse biofilm formation. Compounds will be isolated and characterized with sensitive, high-throughput parallel LC-MS and LC-NMR. Once we define rich sources of compounds, effective assays, and identify new compounds that can block or reverse biofilm formation in Phase I, in Phase II we will use methods optimized here, and further characterize the biological activity of active compounds, to determine their potential for application(s) in protection against biofilm-related damage and disease, and the effectiveness of our methods for discovering valuable compounds. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE