Control of Biofilms by Natural Products

天然产物对生物膜的控制

• 批准号: 6338287
• 负责人: JOHN WILLIAM COSTERTON
• 金额: $ 19.45万
• 依托单位: SEQUOIA SCIENCES, INC.
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-01 至 2002-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6338287
• 关键词: Candida albicans; Pseudomonas aeruginosa; Staphylococcus epidermidis; biological products; high throughput technology; inhibitor /antagonist; liquid chromatography; liquid chromatography mass spectrometry; method development; molecular film; nuclear magnetic resonance spectroscopy; plant extracts

DESCRIPTION (provided by applicant): The importance of Biofilms in chronic and treatment-resistant infectious disease, increasingly is clear. Biofilms also are a major problem in industrial processes. The focus of this proposal is to develop methods for discovering compounds that block and/or reverse biofilm formation, and to discover, and structurally characterize, active compounds. Specifically, we will develop efficient methods to screen extracts and compounds for their ability to regulate biofilms of a gram negative pathogen, and develop and validate new screens with biofilms of gram negative bacteria and fungi. In addition, we will evaluate plants, especially those that live in moist or wet environments, as a possible rich source of novel compounds that block or reverse biofilm formation. Compounds will be isolated and characterized with sensitive, high-throughput parallel LC-MS and LC-NMR. Once we define rich sources of compounds, effective assays, and identify new compounds that can block or reverse biofilm formation in Phase I, in Phase II we will use methods optimized here, and further characterize the biological activity of active compounds, to determine their potential for application(s) in protection against biofilm-related damage and disease, and the effectiveness of our methods for discovering valuable compounds. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE

描述（由申请人提供）：生物膜在慢性和 抗药性传染病的问题越来越明显。生物膜还 是工业过程中的主要问题。本提案的重点是 开发用于发现阻断和/或逆转生物膜的化合物的方法 形成，并发现和结构表征活性化合物。 具体来说，我们将开发有效的方法来筛选提取物， 化合物调节革兰氏阴性病原体生物膜的能力， 开发和验证新的革兰氏阴性菌生物膜筛选 和真菌。此外，我们将评估植物，特别是那些生活在 潮湿或潮湿的环境中，作为一种可能的丰富来源的新化合物， 阻断或逆转生物膜形成。化合物将被分离， 通过灵敏的高通量并行LC-MS和LC-NMR进行表征。一旦 我们定义了丰富的化合物来源，有效的检测方法，并确定了新的 可以在阶段I、阶段II和阶段III中阻断或逆转生物膜形成的化合物 我们将使用这里优化的方法，并进一步表征生物 活性化合物的活性，以确定其应用潜力 在保护免受生物膜相关的损害和疾病， 我们发现有价值化合物的方法 拟议商业应用：不可用