Characterisation of mechanical, physical and biological properties of biofilms under industrial processing conditions

细菌生物膜为微生物提供了对外部环境的物理机械保护，需要使用苛刻的化学品和研磨清洁来控制它们。这种保护机制对各种产品的制造具有广泛的影响，包括那些为卫生（口腔，家庭清洁和织物卫生）而设计的产品，以及它们在家庭中的功效，其中也可以体验生物膜。目前，制造产品可能会遇到细菌生物膜污染的问题，导致生产中断和工业清洁和消毒的显著经济成本。在高流量条件下形成的生物膜尚未从物理和生物学的角度研究响应于这些应力而发生的变化。剪切和流动对生物膜的物理和机械特性的影响将在本项目中进行评估，将这些因素与代谢和细胞活性的变化联系起来。所产生的模型生物膜将用于研究假单胞菌属的遗传、转录组和代谢反应。在制造和清洁过程中施加的应力。这些实验将提供数据，这些数据将为在一系列条件下防止和去除假单胞菌生物膜形成的最有效手段提供信息，以期设计更有效的补救措施。

Bacterial biofilms provide a physico-mechanical protection of microbes to the external environment, requiring the use of harsh chemicals and abrasive cleaning for their control. This protective mechanism has broad impact in the manufacturing of a wide range of products including those designed for hygiene (oral, household cleaning and fabric hygiene) as well as their efficacy within the home, where biofilms can also be experienced. Currently, manufacturing products can be subject to problems with bacterial biofilm contamination leading to production interruption and significant economic cost to industry for cleaning and sanitisation. Biofilms formed under high flow conditions have not been studied in relation to the changes that occur in response to these stresses from a combined physical and biological perspective. The impact of shear and flow on the physical and mechanical properties of the biofilm will be evaluated in this project, relating these factors to changes in metabolism and cellular activity. The model biofilms generated will be used to investigate the genetic, transcriptomic and metabolic responses of Pseudomonas spp. to stresses imposed in manufacturing and cleaning processes. These experiments will provide data which will inform the most effective means to prevent and remove biofilm formation by Pseudomonas in a range of conditions, with a view to designing more effective remed