Core--Cell processing

细胞处理核心的目的是获得和提供人类血细胞，用于作为代谢紊乱基因治疗计划项目资助的一部分进行的研究。通过该核心项目提供的细胞包括外周血干细胞、单核细胞和脐带血细胞。这些细胞将从正常供体或患者大规模获得，并以从供体获得的组合物提供或在实验室中处理以分离亚群如淋巴细胞或CD 34+细胞。细胞处理核心还将提供克隆形成试验服务的体外细胞培养，用于测量和评估经过本计划项目规定的基因转移操作的造血祖细胞。随着项目走向临床应用，该项目将在圣保罗校园的GMP设施，分子和细胞治疗设施提供这些相同的临床试验服务。

The purpose of the Cell Processing Core is to obtain and provide human blood cells for investigations being carried out as part of the Gene Therapy for Metabolic Disorders Program Project Grant. Cells that will be provided through this Core project include peripheral blood stem cells, mononuclear cells, and umbilical cord blood cells. These cells will be obtained on a large scale from either normal donors or patients and provided in the composition obtained from the donor or processed in the laboratory to isolate subpopulations such as lymphocytes or CD34+ cells. The Cell Processing Core will also provide in-vitro cell culture of clonogenic assay services for the measurement and evaluation of hematopoietic progenitor cells that have undergone gene transfer manipulations as specified in this program project. As projects move toward clinical application, the project will supply these same services for clinical trials at the GMP facility on the St. Paul Campus, the Molecular and Cellular Therapeutics Facility.