CORE--ANIMAL MODELING AND OUTCOME

我们建议运营一个核心设施，为匹兹堡大学脑创伤研究中心项目资助（PPG）执行与动物建模和结局评估相关的必要程序。该核心中的设施将由参与本PPG申请中各个提案的以下主要研究者（PI）使用：1）Steven Graham，M.D.，博士，项目#1，“创伤性脑损伤（TBI）中的bcl-2家族基因"，2）Steven Dekosky。项目2美元，“IL-1 β，APP代谢和头部损伤的体温过低”，3）帕特里克Kochanek，医学博士，项目#3，“iNOS和TBI”，4）Edward狄克逊，博士，项目#4“TBI的多巴胺能机制”，和5）Robert Clark，医学博士，项目#5“TBI后PARS激活。“核心设施将解决以下具体目标1）为PPG中所有小鼠和大鼠的拟议实验操作提供手术和麻醉的集中设施，2）为PPG中所有小鼠和大鼠的研究提供统一的损伤（受控皮质撞击[CI]模型和二次损伤CCI模型），并通过进行质量控制分析确保一致性，3）为功能结果参数的标准化评估提供集中设施（运动和认知），4）为组织学结果参数的标准化评估提供集中设施5）为了促进PPG内PI之间的相互作用，并促进与匹兹堡NMR生物医学研究中心（卡内基梅隆大学）的合作研究者的互动，以及6）为在PPG中的大鼠中使用该疗法的所有研究提供标准化的低温方案。成功实现这些特定目标将为研究者提供最佳资源利用，包括使用1）技术人员时间、2）实验室空间、3）设备、4）供应品、5）动物和6）磁共振成像时间。同时，将确保所有小鼠和大鼠研究的质量控制，并为PPG内PI之间的相互作用提供结构化环境。

We propose to operate a core facility that performs the necessary procedures related to Animal Modeling and Outcome Assessment for the University of Pittsburgh Brain Trauma Research Center program project grant (PPG). The facilities in this core will be used by the following principal investigators (PIs) involved in the individual proposals in this PPG application: 1) Steven Graham, M.D., Ph.D., Project #1, "bcl-2 family genes in traumatic brain injury (TBI)", 2) Steven Dekosky., Project $2, "IL-1beta, APP metabolism and hypothermia in head injury", 3) Patrick Kochanek, M.D., Project #3, "iNOS and TBI", 4) Edward Dixon, Ph.D., Project #4 "Dopaminergic mechanisms of TBI," and 5) Robert Clark, M.D., Project #5 "PARS activation after TBI." The core facility will address the following Specific Aims 1) To provide a centralized facility for surgery and anesthesia for all of the proposed experimental manipulations of mice and rats within the PPG, 2) To provide uniform injury for all studies in mice and rats in the PPG (controlled cortical impact [CI] model, and CCI model with secondary insult) and ensure consistency by performing quality control analysis, 3) To provide a centralized facility for the standardized assessment of functional outcome parameters (motor and cognitive) within the PPG, 4) To provide a centralized facility for the standardized assessment of histological outcome parameters (contusion volume and hippocampal neuron counting) within the PPG, 5) To facilitate interaction between the PIs within the PPG, and facilitate interaction with collaborating investigators at the Pittsburgh NMR Center for Biomedical Research (Carnegie Mellon University), and 6) To provide a standardized hypothermia regimen for all studies using this therapy in rats in the PPG. The successful execution of these specific aims will provide for optimal resource utilization by the investigators include the use of 1) technician time, 2) laboratory space, 3) equipment, 4) supplies, 5) animals, and 6) Magnetic Resonance Imaging time. At the same time, quality control will be ensured for all of the studies in mice and rats, and a structured environment for interaction between the PIs within the PPG will be provided.