Modulation of CO2 utilization by Cupriavidus necator H16 for high value chemical production

Cupriavidus necator H16 调节 CO2 利用以进行高价值化学品生产

• 批准号: 1804149
• 金额: --
• 依托单位: University of Nottingham
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2016
• 资助国家: 英国
• 起止时间: 2016 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1804149
• 关键词: Modulation; CO2; utilization; Cupriavidus; necator

CO2 assimilation in R. eutropha, is mediated by Calvin-Benson-Bassham (CBB) cycle encoded by two almost identical cbb operons, one located on the pHG1 megaplasmid and the other on chromosome 2 (Pohlmann et al., 2006). The effective expression of these operons, and in particular the genes encoding the major rate-limiting enzyme, ribulose 1,5-bisphosphate carboxylase/ oxygenase (RubisCO), are crucial. Both operons and are tightly regulated by the cbbR transcriptional activator located at the 5' end of the chromosomal copy of the cbb operon (Kusian and Bowien, 1995). This LysR-type regulatory protein con-trols the expression of the cbb operons by binding to their identical promoter sequences (Dangel and Tabita, 2015). LysR independent, and preferably constitutive transcription of the cbb operon might prove advantageous for enhancing the efficiency of CO2 fixation efficiency of the CBB cycle. To further improve the endogenous CO2 assimilation pathway we bring about the controllable expression of synthetic DNA modules encoding functional carboxysomes for the co-localisation of CAs and RuBisCO molecules with improved CO2-capture capabilities.

欧特罗氏菌中的CO2同化是由Calvin-Benson-Bassham（CBB）循环介导的，该循环由两个几乎相同的CBB操纵子编码，一个几乎相同的CBB操纵子，一个位于phg1 megaplasmid上，另一个位于2号染色体上（Pohlmann等人，2006年）。这些操纵子的有效表达，尤其是编码主要限速酶的基因，核糖1,5-双磷酸羧化酶/加氧酶（Rubisco）至关重要。两项操纵子都由位于CBB操纵子染色体副本5'端的CBBR转录激活剂严格调节（Kusian and Bowien，1995）。这种LYSR型调节蛋白通过结合其相同的启动子序列来控制CBB操纵子的表达（Dangel和Tabita，2015年）。 CBB操纵子的LYSR独立和优选的本构转录可能被证明是提高CBB循环二氧化碳固定效率的效率。为了进一步改善内源性二氧化碳同化途径，我们带来了编码功能性羧化体的合成DNA模块的可控表达，用于CAS和Rubisco分子具有改善的CO2捕获能力。