Advanced Microscopy Shared Resource Facility

描述(由申请人提供)： 这项建议的主要目标是增强和扩大能力 位于西奈山的机构显微镜共享资源设施(SRF) 医学院。这一核心设施的改进将显著 加强NCI资助(和其他NIH资助)的研究工作 整个机构的调查人员。将在以下方面进行改进 三个方面：a)活细胞成像；b)分子定位；c)数字化 图像采集和图像分析。除了增强 设施的能力，总体运营成本将会降低，从而 使初级调查人员更容易(在财务上)接触到该设施。这个 具体目标是：1)提供更多由NCI和其他NIH资助的机会 调查人员使用最先进的仪器和高质量、高成本 通过降低使用费提供有效的显微镜服务；2)教育和 培训NCI资助的研究人员使用先进的显微镜和成像技术 癌症研究中的技术；3)促进NCI资助的显微技术的使用 通过将NCI资助的调查员添加到显微镜SRF咨询中 委员会；4)提高活细胞(活组织)成像能力， 包括多光子激光扫描显微镜，通过提供设备来 支持重要成像(例如细胞培养设备、加热显微镜工作台) 现场并聘请技术人员协助现场细胞/共焦/ 多光子成像；5)提高电子显微镜的易用性 并通过安装数字图像缩短EM结果的周转时间 电子显微镜上的采集系统(cccd摄像机)；以及6)增强 超微结构的免疫定位能力，包括细胞固定， 冷冻替代和冷冻超微切割技术为我们提供了 电子显微镜下的免疫定位。来自16个NCI的代表性研究 资助的实验室(代表MSSM的7个部门)在 这项拨款提案的研究计划。成功的组织、运营、 商业计划已经到位。除了使用培训之外 核心内的活动已经很好地确立了，计划正在制定中 扩大核心(包括研究生院)的教育力度 课程、讲座和多媒体学习材料)。

DESCRIPTION (provided by applicant): The major objective of ths proposal is to enhance and expand the capabilities of an institutional Microscopy Shared Resource Facility (SRF) at Mount Sinai School of Medicine. Improvements to this core facility will significantly enhance the research efforts of NCI-funded (and other NIH-funded) investigators throughout the institution. Improvements will be developed on three fronts: a) live cell imaging; b) molecular localization, and c) digital image acquisition and image analysis. In addition to enhancing the capabilities of the facility, overall operating costs will be lowered, thus making the facility more accessible (financially) to junior investigators. The specific aims are: 1) to provide increased access by NCI- and other NIH-funded investigators to state-of-the-art instrumentation and high quality, cost effective microscopy services by reducing user fees charged; 2) to educate and train NCI-funded investigators in the use of advanced microscopy and imaging techniques in cancer research; 3) to promote NCI-funded use of the Microscopy SRF by adding an NCI-funded investigator to the Microscopy SRF advisory committee; 4) to enhance live-cell (live-tissue) imaging capabilities, including multi-photon laser scanning microscopy, by providing equipment to support vital imaging (e.g. cell culture equipment, heated microscope stages) on site and by hiring a technician to assist with live cell / confocal / multi-photon imaging; 5) to improve user-friendliness of electron microscopy and decrease turn-around time for EM results by installing digital image acquisition system (cCCD camera) on the electron microscope; and 6) to enhance ultrastructural immunolocalization capabilities by including cyro-fixation, freeze-substitution, and cryo-ultramicrotomy techniques to our options for immunolocalization by electron microscopy. Representative studies from 16 NCI funded labs (representing 7 departments at MSSM) have been highlighted in the Research Plan of this grant proposal. Successful organizational, operational, and business plans are already in place. In addition to the use training activities already well established within the core, plans are being developed to expand the educational efforts of the core (including a graduate school course, lectures, and multi-media learning materials).