Acquisition of a spinning disc confocal microscope for a core microscopy facility

为岩心显微镜设施购置转盘共焦显微镜

• 批准号: 7795500
• 负责人: SCOTT C. HENDERSON
• 金额: $ 48.31万
• 依托单位: VIRGINIA COMMONWEALTH UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-27 至 2011-05-26
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7795500
• 关键词: Acute; Biomedical Engineering; Cells; Cellular biology; Collaborations; Consultations; Core Facility; Data Collection; Equipment; Fluorescence; Funding; Funding Applicant; Housing; Image; Image Analysis; Instruction; Lasers; Life; Microbiology; Microscope; Microscopic; Microscopy; Neurobiology; Organism; Pharmacology; Physiology; Preparation; Productivity; Reporting; Research; Research Personnel; Research Support; Resolution; Resource Sharing; Sampling; Scanning; Scanning Transmission Electron Microscopy Procedures; Services; Slice; Speed; Staging; System; Tissues; Universities; Virginia; Zebrafish; charge coupled device camera; instrument; instrumentation; medical schools; meetings; multi-photon; programs; virology

DESCRIPTION (provided by applicant): Funds are requested to acquire a Zeiss Cell Observer SD (spinning disc) confocal microscope to support the research efforts of multiple NIH-funded investigators at Virginia Commonwealth University (VCU). This live cell / live tissue imaging system is to be housed in a well-established, well-used and highly productive microscopy shared resource facility in the School of Medicine at VCU. The VCU Microscopy Facility is available to all VCU investigators and serves diverse research programs. The facility is directed by the PI, who has expertise in multiple aspects of microscopic imaging. Staff is available for consultation, instruction, assistance and collaboration. The requested equipment will help meet increasing research demands that cannot be fulfilled by the existing equipment in the Facility; namely, the ability to collect high resolution, high contrast confocal images at rapid image acquisition rates. Many research questions have been developed that require a higher temporal resolution and cannot be answered the relatively slow laser-scanning (point scanning) confocal systems that are presently in the facility. The requested Zeiss Cell Observer SD spinning disc confocal microscope is configured around an AxioObserver Z1 inverted stand and equipped with a Yokogawa CSU-X1A spinning disc unit, a Photometrics QuantEM 512SC cooled CCD camera (for high speed, high sensitivity imaging), an AxioCam MRm (for high resolution imaging), a high resolution piezo driven Z stage (for rapid 3D data collection) and a stage incubation system (to support live cell / live tissue imaging). This instrument will be used to image a variety of sample types including cells in culture, acute tissue slices, organotypic slice cultures and organisms (e.g. zebra fish). During the past year (2008), 83 different labs, representing 222 investigators from 23 different departments at VCU have used the Microscopy Facility and its services (widefield fluorescence, confocal, multi-photon, TIRF, atomic force/scanning probe, transmission & scanning electron microscopy, image analysis and sample preparation). During 2008, the laser scanning confocal microscope was used by 104 users from 49 labs, representing 17 departments. The projects conducted by these researchers represent a broad range of studies (cell biology, microbiology, neurobiology, bioengineering, virology, pharmacology, physiology, etc.). Representative studies of major users (from 9 labs; 5 departments) have been highlighted in this proposal. Included are research reports from current users of the Microscopy Facility (Akbarali, Fuss, Hauser, Knapp, Povlishock, Ratz) as well as from 3 other investigators (Block, Lister, Tombes) who require the requested instrumentation to advance their research programs. The acquisition of a rapid imaging live cell confocal system will have a major impact on research at VCU as it will enhance the research capability and productivity of a well used core facility and will attract additional users (whose needs currently cannot be met by the available equipment).

描述(由申请人提供)：申请资金购买蔡司细胞观察者SD(旋转圆盘)共聚焦显微镜，以支持弗吉尼亚联邦大学(VCU)由NIH资助的多名研究人员的研究努力。这个活细胞/活组织成像系统将被安置在弗吉尼亚大学医学院的一个成熟、使用良好和高效的显微镜共享资源设施中。VCU显微镜设施可供所有VCU调查人员使用，并为各种研究项目提供服务。该设施由PI领导，他在显微成像的多个方面拥有专业知识。工作人员可以进行咨询、指导、协助和协作。所需设备将有助于满足该设施现有设备无法满足的日益增长的研究需求，即以快速图像采集速度收集高分辨率、高对比度共焦图像的能力。已经提出了许多需要更高时间分辨率的研究问题，而目前该设施中相对较慢的激光扫描(点扫描)共焦系统无法回答这些问题。所要求的蔡司细胞观察者SD旋转盘共焦显微镜围绕Axio观察者Z1倒置支架配置，并配备了横河CSU-X1A型旋转盘单元、光度测量QuantEM 512SC冷却CCD摄像机(用于高速、高灵敏度成像)、AxioCam MRM(用于高分辨率成像)、高分辨率压电驱动Z Stage(用于快速3D数据采集)和舞台孵化系统(以支持活细胞/活组织成像)。该仪器将用于对各种类型的样本进行成像，包括培养细胞、急性组织切片、器官型切片培养物和生物体(例如斑马鱼)。在过去的一年(2008年)，来自VCU 23个不同部门的222名研究人员的83个不同实验室使用了显微镜设备及其服务(广域荧光、共焦、多光子、TIRF、原子力/扫描探头、透射和扫描电子显微镜、图像分析和样品制备)。2008年期间，来自17个部门的49个实验室的104名用户使用了激光扫描共聚焦显微镜。这些研究人员开展的项目涉及广泛的研究领域(细胞生物学、微生物学、神经生物学、生物工程、病毒学、药理学、生理学等)。该提案强调了对主要用户(来自9个实验室；5个部门)的代表性研究。包括来自显微镜设备的当前用户(Akbarali，Fuss，Hauser，Knapp，Povlishock，Ratz)以及其他3名研究人员(Block，Lister，Tombes)的研究报告，他们需要所要求的仪器来推进他们的研究计划。购置快速成像活细胞共焦系统将对VCU的研究产生重大影响，因为它将提高一个使用得很好的核心设施的研究能力和生产率，并将吸引更多的用户(其需求目前无法由现有设备满足)。