SCAN ELECTRON MICROSCOPE: ROLE OF MICROGLIA IN INFECTION OF BRAIN, AIDS DEMENTIA

扫描电子显微镜：小胶质细胞在大脑感染、艾滋病痴呆中的作用

• 批准号: 7335212
• 负责人: SCOTT C. HENDERSON
• 金额: $ 1.51万
• 依托单位: VIRGINIA COMMONWEALTH UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7335212
• 关键词: AIDS; brain; dementia; infection; microglia; microscopy; role; university

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. Regarding AIDS DESCRIPTION (provided by applicant): We are requesting funds to acquire a scanning electron microscope to upgrade a well-established microscopy shared resource facility. This facility is available to all Virginia Commonwealth University (VCU) investigators and serves diverse research programs. This equipment will replace the facility's 18-year old SEM that is failing. Furthermore, the requested equipment will help to meet the increasing research demands that are not fulfilled presently by the existing equipment in the Facility. In particular, there is a need for a technologically up-to-date, user-friendly scanning electron microscope. Many research questions have been developed that cannot be answered by the failing instrument that is currently in the facility. The proposed equipment for purchase is a Zeiss EVO 50 XVP scanning electron microscope. This instrument can be used to image non-coated, non-conductive, and "wet" samples under variable pressure conditions. Thus, samples that are susceptible to preparation artifacts may be imaged with minimal (or no) pretreatment (i.e. non-conductive samples, non-coated samples, hydrated samples). Functioning as a new shared resource for the institution, during 2004, 36 different labs, representing 89 investigators from 17 different departments within the university have used the VCU - Microscopy Facility and its services (fluorescence microscopy, confocal microscopy, and transmission electron microscopy). The projects conducted by these researchers represent a broad range of studies (cell biology, microbiology, neurobiology, bioengineering, orthodontics). Many of these projects are integral to thesis research of graduate students in the Ph.D. program. Representative studies of NIH-funded major users (from 7 labs; 3 departments) have been highlighted in the Research Activities section of this grant proposal. Included are research reports from current users of the Microscopy Facility (Fuss, Ohman, Povlishock, Simpson, Bowlin, Cabral, and Wayne). Work from 2 other labs (Shroff, and Tufekci) in an additional department is also presented as representative of minor users generating preliminary data for future research grant applications. Improvements to the facility will have a major impact on research at VCU: 1) The proposed equipment will enhance the research capability and productivity of a well used core facility; 2) The new equipment will attract even more users (whose needs currently cannot be met by the available equipment). 3) A course in emerging techniques of imaging currently under development for graduate students and post-doctoral fellows will utilize the SEM and will add to the user base of the Microscopy Facility. 4) The histology courses of the medical, dental, nursing, and physical therapy schools will have access to digital SEM images that may be integrated into their all-digital laboratories.

这个子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是用于拨款的，而不一定是用于调查人员的机构。关于艾滋病描述(由申请人提供)：我们正在申请资金，以购买一台扫描电子显微镜，以升级一个完善的显微镜共享资源设施。这一设施向所有弗吉尼亚联邦大学(VCU)的调查人员开放，并为不同的研究项目提供服务。这台设备将取代该设施已有18年历史的扫描电子显微镜，后者正在发生故障。此外，所要求的设备将有助于满足不断增加的研究需求，而这些需求目前尚不是该设施现有设备所能满足的。尤其需要一种技术上最新的、用户友好的扫描电子显微镜。许多研究问题已经被开发出来，而目前该设施中的故障仪器无法回答这些问题。拟购买的设备是蔡司EVO 50XVP扫描电子显微镜。该仪器可用于在可变压力条件下对非涂层、非导电和“湿”样品进行成像。因此，易受制备伪影影响的样品可在最小(或无)预处理的情况下成像(即，非导电样品、非涂层样品、水合样品)。作为该机构的一个新的共享资源，2004年期间，36个不同的实验室，代表来自该大学17个不同系的89名研究人员，使用了VCU显微镜设施及其服务(荧光显微镜、共聚焦显微镜和透射电子显微镜)。这些研究人员进行的项目代表了广泛的研究范围(细胞生物学、微生物学、神经生物学、生物工程、正畸)。这些项目中的许多都是博士研究生论文研究的组成部分。NIH资助的主要用户(来自7个实验室；3个部门)的代表性研究在这项拨款提案的研究活动部分得到了强调。其中包括来自显微镜设备当前用户(Fuss、Ohman、Povlishock、Simpson、Bowlin、Cabral和Wayne)的研究报告。另外一个部门的另外两个实验室(Shroff和Tufekci)的工作也作为少数用户的代表，为未来的研究资助申请生成了初步数据。设施的改进将对VCU的研究产生重大影响：1)拟议的设备将提高一个使用良好的核心设施的研究能力和生产率；2)新设备将吸引更多的用户(现有设备目前无法满足他们的需求)。3)目前正在为研究生和博士后研究员开发的一门新兴成像技术课程将利用扫描电子显微镜，并将增加显微镜设备的用户基础。4)医学、牙科、护理和理疗学院的组织学课程将可以访问数字扫描电子显微镜图像，这些图像可能会集成到他们的全数字实验室中。