MECHANISTIC ANALYSIS OF SITE DIRECTED MUTANT NUCLEASE ENZYMES
MECHANISTIC ANALYSIS OF SITE DIRECTED MUTANT NUCLEASE ENZYMES
批准号:
6470648
负责人:
BINGHUI SHEN
金额:
$12.12万
依托单位国家:
美国
项目类别:
财政年份:
2001
资助国家:
美国
项目状态:
已结题
起止时间:
2001-07-01 至 2002-06-30
中文摘要
本研究的主要目的是了解
一个新的生物体的结构和功能之间的相互关系
鉴定的核酸酶人瓣核酸内切酶-1(FEN-1)
DNA修复 目前的生物化学和遗传学数据表明
FEN-1是结构特异性内切核酸酶。 它可能会起到关键作用,
在DNA复制、修复和重组中的作用。 然而,在这方面,
没有足够的证据表明这类核酸酶可以
为它们独特的生物化学和
生理功能及其结构和功能
关系。 我们对生化分析的初步数据
结构域的研究表明,在人类中存在三个功能结构域,
FEN-1蛋白负责DNA底物结合的功能,
催化、蛋白质-蛋白质相互作用和核定位。 的
研究旨在建立详细的结构
了解DNA底物识别的催化中心,
结合和裂解。 并分析其结构元素
负责蛋白质-蛋白质相互作用和核定位。
我们将通过现场指导检查功能改变
每个功能结构域的诱变。 突变蛋白质将
通过我们新开发的动态流式细胞术表征,
生化分析和晶体结构分析,以及绿色
荧光驱动显微镜。
英文摘要
The major objective of the proposed research is to understand the
interrelationship between the structure and function of a newly
identified nuclease human flap endonuclease-1 (FEN-1) in the context
of DNA repair. Current biochemical and genetic data have demonstrated
that FEN-1 is a structure specific endonuclease. It may play critical
roles in DNA replication, repair, and recombination. However,
sufficient evidence is not available for this class of nucleases to
establish a structural basis for their unique biochemical and
physiological functions and their structural and functional
relationships. Our preliminary data on the analysis of biochemical
domains has suggested that there are three functional domains in human
FEN-1 protein responsible for the functions of DNA substrate binding,
catalysis, protein-protein interaction, and nuclear localization. The
proposed research is designed to establish the detailed structural
understanding of the catalytic cen ter for DNA substrate recognition,
binding and cleavage. and to analyze the structural elements
responsible for protein-protein interaction and nuclear localization.
We will examine functional alterations through site-directed
mutagenisis of each functional domain. The mutant proteins will be
characterized by our newly developed kinetic flow cytometry,
biochemical assays and crystallographic structural analysis, and green
fluorescence driven microscopy.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Okazaki fragment maturation: mutagenesis and cell survival
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批准号:10636417
-
项目类别:
-
资助金额:$54.78万
-
财政年份:2023
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负责人:BINGHUI SHEN
-
依托单位:
DNA repair gene mutations and prostate cancer
-
批准号:10307594
-
项目类别:
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资助金额:$68.38万
-
财政年份:2019
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负责人:BINGHUI SHEN
-
依托单位:
DNA repair gene mutations and prostate cancer
-
批准号:10064136
-
项目类别:
-
资助金额:$69.78万
-
财政年份:2019
-
负责人:BINGHUI SHEN
-
依托单位:
DNA repair gene mutations and prostate cancer
-
批准号:9883610
-
项目类别:
-
资助金额:$71.68万
-
财政年份:2019
-
负责人:BINGHUI SHEN
-
依托单位:
DNA repair gene mutations and prostate cancer
-
批准号:10529297
-
项目类别:
-
资助金额:$68.38万
-
财政年份:2019
-
负责人:BINGHUI SHEN
-
依托单位:
DNA Damage Response and Oncogenic Signaling
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批准号:10577782
-
项目类别:
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资助金额:$23.46万
-
财政年份:2016
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负责人:BINGHUI SHEN
-
依托单位:
DNA Damage Response and Oncogenic Signaling
-
批准号:10332432
-
项目类别:
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资助金额:$23.37万
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财政年份:2016
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负责人:BINGHUI SHEN
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依托单位:
Lung and other cancer etiological model of BER gene polymorphisms
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批准号:8103282
-
项目类别:
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资助金额:$17.51万
-
财政年份:2010
-
负责人:BINGHUI SHEN
-
依托单位:
Lung and other cancer etiological model of BER gene polymorphisms
-
批准号:7990964
-
项目类别:
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资助金额:$21.66万
-
财政年份:2010
-
负责人:BINGHUI SHEN
-
依托单位:
Role of Nucleases in RNA Primer Removal and Mutagenesis
-
批准号:7809910
-
项目类别:
-
资助金额:$50.55万
-
财政年份:2009
-
负责人:BINGHUI SHEN
-
依托单位:
MECHANISTIC ANALYSIS OF SITE DIRECTED MUTANT NUCLEASE ENZYMES
-
批准号:6327941
-
项目类别:
-
资助金额:$0.31万
-
财政年份:2000
-
负责人:BINGHUI SHEN
-
依托单位:
NUCLEASES IN RNA PRIMER REMOVAL AND MUTAGENESIS
-
批准号:6514394
-
项目类别:
-
资助金额:$19.01万
-
财政年份:1999
-
负责人:BINGHUI SHEN
-
依托单位:
NUCLEASES IN RNA PRIMER REMOVAL AND MUTAGENESIS
-
批准号:6377605
-
项目类别:
-
资助金额:$18.46万
-
财政年份:1999
-
负责人:BINGHUI SHEN
-
依托单位:
Dynamic functions of DNA2 counteract DNA replication stresses and tumorigenesis
-
批准号:8630919
-
项目类别:
-
资助金额:$29.4万
-
财政年份:1999
-
负责人:BINGHUI SHEN
-
依托单位:
Dynamic functions of DNA2 counteract DNA replication stresses and tumorigenesis
-
批准号:9913472
-
项目类别:
-
资助金额:$33.34万
-
财政年份:1999
-
负责人:BINGHUI SHEN
-
依托单位:
Dynamic functions of DNA2 counteract DNA replication stresses and tumorigenesis
-
批准号:8777944
-
项目类别:
-
资助金额:$29.4万
-
财政年份:1999
-
负责人:BINGHUI SHEN
-
依托单位:
Role of Nucleases in RNA Primer Removal and Mutagenesis
-
批准号:7083614
-
项目类别:
-
资助金额:$27.63万
-
财政年份:1999
-
负责人:BINGHUI SHEN
-
依托单位:
Role of Nucleases in RNA Primer Removal and Mutagenesis
-
批准号:6902659
-
项目类别:
-
资助金额:$28.29万
-
财政年份:1999
-
负责人:BINGHUI SHEN
-
依托单位:
NUCLEASES IN RNA PRIMER REMOVAL AND MUTAGENESIS
-
批准号:6457243
-
项目类别:
-
资助金额:$6.37万
-
财政年份:1999
-
负责人:BINGHUI SHEN
-
依托单位:
Role of Nucleases in RNA Primer Removal and Mutagenesis
-
批准号:8387769
-
项目类别:
-
资助金额:$24.32万
-
财政年份:1999
-
负责人:BINGHUI SHEN
-
依托单位:
国内基金
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基于Pan-genome技术的沙门氏菌血清型特异性基因挖掘、功能分析及分子鉴定
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批准号:21242003
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项目类别:专项基金项目
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资助金额:10.0万元
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批准年份:2012
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负责人:昌军
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依托单位:
基于Pan-genome技术探究问号钩端螺旋体不同血清型致病性差异的遗传基础
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批准年份:2011
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负责人:郭晓奎
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