PROTEIN KINASE CATALYTIC SUBUNIT & CELL CYCLE CHECK POINTS IN DAMAGED CELLS

多参数流式细胞术，包括BrdU含量分析， 用来确定DNA依赖的蛋白激酶 催化亚基(DNA-PKcs)在细胞周期停滞中的作用 对DNA损伤的反应。为了调查缺乏……的影响 DNA-PKcs在G1期细胞周期检查点，DNA-PKcs+/-和-/-小鼠 胚胎成纤维细胞以6.0Gy射线照射，然后连续照射 标有BrdU的。到照射后6小时，细胞从 DNA-PKcs+/-和-/-培养中的S期均表明诱导 在G1检查站被逮捕。初步结果显示， G1期停滞程度与DNA-PKcs+/-和 --文化。未来的研究将包括连续的标签和 BrdU和随后较长时间间隔的细胞检查 辐射以检查细胞周期恢复模式。

Multiparameter flow cytometry, including BrdU content analysis, is being used to determine whether the DNA-dependent protein kinase catalytic subunit (DNA-PKcs) has a role in cell cycle arrest in response to DNA damage. To investigate the effect of the absence of DNA-PKcs on the G1 cell cycle checkpoint, DNA-PKcs +/- and -/- mouse embryonic fibroblasts were irradiated at 6.0 Gy and then continuously labeled with BrdU. By 6 h post-irradiation, cells were depleted from the S phase in both DNA-PKcs +/- and -/- cultures indicating induction of arrest at a G1 checkpoint. Preliminary results show that the extent of G1 arrest was comparable for the DNA-PKcs +/- and -/-cultures. Future studies will involve continuous labeling with BrdU and examination of the cells at longer time intervals following radiation to examine the cell cycle recovery patterns.