CAFFEINE & STAUROSPORINE: CELL CYCLE & CYCLIN B1 EXPRESSION IN GAMMA IRRAD CELLS

使用流式细胞术比较咖啡因和 星形孢菌素对辐射诱导的 G2 期阻滞和细胞周期蛋白 B1 的影响 在正常人皮肤成纤维细胞 (HSF) 和 HeLa 细胞中表达 在 6.0 Gy 的伽马辐射之后。 咖啡因和星形孢菌素 在减弱辐射引起的增加方面同样有效 细胞周期蛋白B1的表达和G2的延长在同步和 异步 HeLa 细胞群。 咖啡因也能有效 在受辐射的 HSF 细胞中具有相似的程度，但星形孢菌素 不能有效释放辐射诱导的 G2 阻滞，但确实有效 降低这些细胞群中的细胞周期蛋白 B1 水平。 这些结果 表明星形孢菌素作用于细胞周期的不同途径 控制正常和转化细胞。

Flow cytometry was used to compare effects of caffeine and staurosporine on the radiation-induced G2-phase arrest and cyclin B1 expression in normal, human skin fibroblast (HSF) cells and HeLa cells following 6.0 Gy of gamma radiation. Caffeine and staurosporine were equally effective in attenuating both radiation induced increase in cyclin B1 expression and the prolongation of G2 in synchronous and asynchronous HeLa cell populations. Caffeine was also effective to a similar extent in irradiated HSF cells but staurosporine was ineffective in releasing the radiation-induced G2 block but did decrease cyclin B1 levels in those cell populations. These results suggest that staurosporine acts on different pathways of cell cycle control in normal and transformed cells.