CAFFEINE & STAUROSPORINE: CELL CYCLE & CYCLIN B1 EXPRESSION IN GAMMA IRRAD CELLS

咖啡因

• 批准号: 6470670
• 负责人: PADMA NARAYANAN
• 金额: $ 12.12万
• 依托单位: UNIVERSITY OF CALIF-LOS ALAMOS NAT LAB
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-07-01 至 2002-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6470670
• 关键词: biomedical resource; human tissue; proteins; technology /technique

Flow cytometry was used to compare effects of caffeine and staurosporine on the radiation-induced G2-phase arrest and cyclin B1 expression in normal, human skin fibroblast (HSF) cells and HeLa cells following 6.0 Gy of gamma radiation. Caffeine and staurosporine were equally effective in attenuating both radiation induced increase in cyclin B1 expression and the prolongation of G2 in synchronous and asynchronous HeLa cell populations. Caffeine was also effective to a similar extent in irradiated HSF cells but staurosporine was ineffective in releasing the radiation-induced G2 block but did decrease cyclin B1 levels in those cell populations. These results suggest that staurosporine acts on different pathways of cell cycle control in normal and transformed cells.

流式细胞术用于比较咖啡因和 星形孢菌素对辐射诱导的G2期阻滞和细胞周期蛋白B1的影响 在正常人皮肤成纤维细胞（HSF）和HeLa细胞中的表达 在6.0戈伊的伽马辐射后。 咖啡因和星形孢菌素 同样有效地衰减辐射引起的 细胞周期蛋白B1的表达和G2期的延长， 异步HeLa细胞群。 咖啡因也能有效地 在照射的HSF细胞中类似程度，但星形孢菌素 在释放辐射诱导的G2阻滞方面无效，但 降低这些细胞群中的细胞周期蛋白B1水平。 这些结果 表明星形孢菌素作用于细胞周期不同途径 在正常和转化细胞中的对照。