Mass Spectrometer

描述（由申请人提供）：本提案要求资金 购买Micromass Q-TOF Ultima Global Hybrid Quadrupole/orthogonal 光谱仪及其操作所需的附件。这种乐器被 选择它是因为它提供了功能（通过要安装的选项） MALDI和Q-TOF仪器的质量， Q-TOF的分辨率、质量准确度或电喷雾灵敏度。这 要求对现有质谱仪进行升级 在WVU可用的工具。附近没有类似的仪器 设施该仪器将成为蛋白质组学/质谱分析的核心仪器。 光谱设施，将支持几个正在进行的NIH项目（表1） 以及COBRe补助金（RR 16440），将支持五名初级调查员。 虽然COBRe蛋白质组学/MS核心将由可用的Finnigan LCQ-DECA MS，该仪器有许多限制（详述如下）， 不适合长期使用。因此，这一升级对于 继续利用蛋白质组学/MS核心设施。除了有 研究人员列出证明这一工具，预计随着 对科学中蛋白质组学的兴趣日益增加，核心设施将 看到越来越多的使用。

DESCRIPTION (provided by applicant): This proposal requests the funds to purchase a Micromass Q-TOF Ultima Global Hybrid Quadrupole/orthogonal spectrometer and accessories necessary for its operation. This instrument was chosen because it provides the capabilities (through options to be installed) of both a MALDI as well as a Q-TOF instrument without compromising the mass resolution, mass accuracy or electrospray sensitivity of the Q-TOF. This instrument is requested as a upgrade to the current mass spectrometer instruments available at WVU. No comparable instruments are available in nearby facilities. This instrument will be the core instrument for a proteomics/mass spectrometry facility that will support several on-going NIH projects (Table 1) as well as a COBRe grant (RR16440) that will support five junior investigators. While the COBRe proteomics/MS core will be initiated with an available Finnigan LCQ -DECA MS, this instrument has many limitations (detailed below) that make it unsuitable for long-term use. Thus, this upgrade is essential to the continued utility of the proteomics/MS core facility. In addition to the investigators listed to justify this instrument, it is anticipated that with the increasing interest in proteomics in science, that the core facility will see more and more use.