X-Ray Crystallography Imaging Plate Detector

X 射线晶体学成像板探测器

• 批准号: 6440166
• 负责人: HARRY F NOLLER
• 金额: $ 26.4万
• 依托单位: UNIVERSITY OF CALIFORNIA SANTA CRUZ
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-01 至 2003-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6440166
• 关键词: X ray crystallography; biomedical equipment purchase; image processing; structural biology

DESCRIPTION(provided by applicant): This proposal requests funds to purchase an R-AXIS IV++ imaging plate X-ray detector for the structural biologists in the Departments of Chemistry and Molecular, Cellular and Developmental Biology at the University of California, Santa Cruz. The current detector (R-AXIS IIC) has been nonfunctional for over 10 months and repeated efforts to locate the cause of the malfunction both by members of the structural biology group at UCSC as well as the manufacturers have been unsuccessful. There are currently three crystallography laboratories at UCSC pursuing a diverse range of exciting research projects. The projects under investigation include (1). the 70S ribosome, complexes with mRNA and tRNA, complexes of the ribosome with trigger factor, ribosome recycling factor and release factors, (2) complexes of the hammerhead ribozyme with transition state analogues, (3) the hairpin ribozyme, (4) RNaseP, (5) flavoenzymes, and (6) multifunctional enzymes involved in substrate channeling. The projects range from those for which crystals are already available to those where crystallization screening is underway to either improve current crystals or to identify new crystallization conditions. For all projects under study, progress is seriously hampered by the fact that there is no functioning in-house X-ray detector currently available. We propose to purchase the R-AXIS IV++ imaging plate detector and a number of accessory items including a 2 theta stage and confocal optical system in order to have a state-of-the-art system that can be used for routine screening of crystals as well as high-resolution data collection.

描述（由申请人提供）：该提案要求资金购买 R-AXIS IV++ 成像板 X 射线探测器，适用于结构生物学家 化学系和分子、细胞和发育生物学系 加州大学圣克鲁斯分校。电流检测器（R-AXIS IIC）具有 超过10个月无法使用并反复努力查找原因 UCSC 结构生物学小组成员的故障 以及制造商都没有成功。目前有三个 UCSC 的晶体学实验室致力于各种令人兴奋的研究 研究项目。正在调研的项目包括（1）。 70年代 核糖体，与 mRNA 和 tRNA 的复合物，核糖体与触发器的复合物 因子、核糖体回收因子和释放因子，(2) 的复合物 具有过渡态类似物的锤头核酶，(3) 发夹核酶， (4) RNaseP、(5) 黄素酶和 (6) 参与的多功能酶 底物沟道。这些项目包括晶体的项目 已经可供那些正在进行结晶筛选的人使用 要么改进现有晶体，要么确定新的结晶条件。 对于所有正在研究的项目来说，进展都受到以下事实的严重阻碍： 目前没有可用的内部 X 射线探测器。我们建议 购买 R-AXIS IV++ 成像板探测器和一些配件 项目包括 2 Theta 平台和共焦光学系统，以便具有 最先进的系统，可用于晶体的常规筛选 以及高分辨率数据采集。