X-Ray Crystallography Imaging Plate Detector

描述(由申请人提供)：本建议书申请资金购买 R轴IV++成像板X射线探测器 化学与分子、细胞与发育生物学系 加州大学圣克鲁斯分校。电流检测器(R轴IIC)具有 10个多月来一直无法正常工作，并反复努力查找原因 加州大学洛杉矶分校结构生物学小组成员发生的故障 由于制造商一直没有取得成功。目前有三个 加州大学圣迭戈分校的结晶学实验室追求一系列令人兴奋的 研究项目。正在调查的项目包括(1)。70年代 核糖体、与mRNA和tRNA的复合体、核糖体与触发物的复合体 因子，核糖体循环因子和释放因子，(2) 具有过渡态类似物的锤头状核酶，(3)发夹状核酶， (4)RNaseP，(5)黄素酶，(6)参与 衬底沟道。这些项目的范围从水晶是 已经提供给那些正在进行结晶筛选的人 要么改进现有的晶体，要么确定新的结晶条件。 对于所有正在研究的项目，进展受到以下事实的严重阻碍 目前还没有可用的内部X射线探测器。我们建议 购买R-AXIS IV++成像板探测器和一些附件 包括2theta工作台和共焦光学系统在内的项目，以便具有 最先进的系统，可用于晶体的常规筛选，如 以及高分辨率数据收集。

DESCRIPTION(provided by applicant): This proposal requests funds to purchase an R-AXIS IV++ imaging plate X-ray detector for the structural biologists in the Departments of Chemistry and Molecular, Cellular and Developmental Biology at the University of California, Santa Cruz. The current detector (R-AXIS IIC) has been nonfunctional for over 10 months and repeated efforts to locate the cause of the malfunction both by members of the structural biology group at UCSC as well as the manufacturers have been unsuccessful. There are currently three crystallography laboratories at UCSC pursuing a diverse range of exciting research projects. The projects under investigation include (1). the 70S ribosome, complexes with mRNA and tRNA, complexes of the ribosome with trigger factor, ribosome recycling factor and release factors, (2) complexes of the hammerhead ribozyme with transition state analogues, (3) the hairpin ribozyme, (4) RNaseP, (5) flavoenzymes, and (6) multifunctional enzymes involved in substrate channeling. The projects range from those for which crystals are already available to those where crystallization screening is underway to either improve current crystals or to identify new crystallization conditions. For all projects under study, progress is seriously hampered by the fact that there is no functioning in-house X-ray detector currently available. We propose to purchase the R-AXIS IV++ imaging plate detector and a number of accessory items including a 2 theta stage and confocal optical system in order to have a state-of-the-art system that can be used for routine screening of crystals as well as high-resolution data collection.