A High-Throughput Crystallization Screening System for the UCSC Macromolecular X-

UCSC 大分子 X- 的高通量结晶筛选系统

• 批准号: 7390440
• 负责人: HARRY F NOLLER
• 金额: $ 25.01万
• 依托单位: UNIVERSITY OF CALIFORNIA SANTA CRUZ
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-02-15 至 2009-02-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7390440
• 关键词: Biological Sciences; Biomedical Research; California; Communities; Condition; Crystallization; Culicidae; Doctor of Philosophy; Equipment; Funding; Housing; Image; Institutes; Liquid substance; Manuals; Methods; Microscope; Operative Surgical Procedures; Range; Research; Robot; Robotics; Roentgen Rays; Screening procedure; System; Training; Wages; X-Ray Crystallography; high throughput screening; instrument; instrumentation; research study; structural biology; thymidine 5' -triphosphate

DESCRIPTION (provided by applicant): The macromolecular X-ray crystallography group at UCSC has developed a proposal to acquire automated liquid handling robotics and an imaging microscope for crystallization screening. The robot and imaging system allow for high-throughput screening of macromolecular crystallization conditions, while reducing the amount of purified material required for these experiments. The instrumentation will permit our research groups to screen ranges of crystallization conditions that would be impractical using current manual methods. Specifically, we are requesting funds to purchase a TTP Labtech mosquito. crystallization robot and CrystalPro HT-80D microscope imaging system. Both instruments are robust and offer ease of use which make them ideally suited for operation in a multi-user facility. They will be housed in space that is adjacent to the current macromolecular X-ray facility. A PhD-level facility manger will maintain the equipment and train users. We have obtained institutional support from the Division of Physical and Biological Science (PBSci) at UCSC and the California Institute for Quantitative Biomedical Research (QB3) in terms of contributing funds towards the salary of the facility manager. Furthermore, PBSci is providing space to house the equipment. The proposed instrumentation will greatly enhance the macromolecular X-ray crystallography facility at UCSC, enabling us to create a fully modern platform for carrying out structural analysis of biomolecules and to increase the accessibility of structural biology to the UCSC biomedical research community.

描述(由申请人提供)：加州大学圣迭戈分校的大分子X射线结晶学小组已经提出了一项计划，以获得自动化液体处理机器人和用于结晶筛选的成像显微镜。机器人和成像系统允许高通量筛选大分子结晶条件，同时减少这些实验所需的纯化材料的数量。该仪器将允许我们的研究小组筛选结晶条件的范围，这些条件使用当前的手动方法是不切实际的。具体地说，我们正在申请资金购买TTP LabTech蚊子。结晶机器人和CrystalPro HT-80D显微镜成像系统。这两种仪器都坚固耐用，易于使用，这使得它们非常适合在多用户设施中操作。它们将被安置在与目前的大分子X射线设备相邻的空间中。一名博士级别的设施经理将维护设备并培训用户。我们已经从加州大学伯克利分校物理和生物科学部(PBSci)和加州定量生物医学研究所(QB3)获得了机构支持，为设施经理的工资提供了捐款。此外，PBSci正在为这些设备提供空间。拟议的仪器将极大地增强加州大学伯克利分校的大分子X射线结晶学设施，使我们能够创建一个完全现代化的平台，进行生物分子的结构分析，并增加结构生物学对加州大学生物医学研究社区的可及性。