RNA EDITING IN PLANT ORGANELLES

RNA编辑改变了预测的细胞核或细胞器基因产物， 许多生物，从原生动物到人类。这种独特的形式 RNA加工的复杂性使生物体在生产 信使和结构RNA和蛋白质。 为 在一些植物和动物的细胞器中，RNA编辑可以被视为一种 基因的修正机制，否则不能表达或 会编码异常产物 RNA编辑有时也用于 允许生产具有不同功能或性质的蛋白质 从一个基因。 在人类中，参与脂质代谢的基因的转录本 新陈代谢和神经系统功能是那些经历基础 修改. 碱基修饰RNA编辑的基本方面 将通过研究C-to-U RNA编辑在质体中的发现， 被子植物 与其他发生RNA编辑的细胞器不同 质体可以通过故意改变的编辑位点进行转化， 分析催化和识别所需的顺式作用元件 的基础进行修改。 突变筛查将识别核或 影响编辑位点识别和编辑的细胞器基因 效率 利用从变换分析获得的信息， 可以设计底物RNA以鉴定编辑的组分， 与目标转录物相关的装置。 拟议研究 这将有助于我们更好地理解其他形式的RNA加工， 特别是tRNA、mRNA和rRNA的作用机制 修改和编辑。

RNA editing alters the predicted nuclear or organelle gene products from many organisms, ranging from protozoans to humans. This distinctive form of RNA processing gives organisms unique flexibility in producing messenger and structural RNAs and proteins from DNA genomes. For organelles of some plants and animals, RNA editing can be viewed as a correction mechanism for genes that otherwise could not be expressed or would encode aberrant products. RNA editing also sometimes serves to allow the production of proteins with diverse functions or properties from a single gene. In humans, transcripts of genes involved in lipid metabolism and nervous system function are among those that undergo base modifications. Fundamental aspects of base modification RNA editing will be uncovered by studies of C-to-U RNA editing in plastids of angiosperm plants. Unlike other organelles in which RNA editing occurs plastids can be transformed with deliberately altered editing sites to analyze the cis-acting elements necessary for catalysis and recognition of bases for modification. Mutant screens will identify nuclear or organelle genes which affect editing site recognition and editing efficiency. With information obtained from transformation analysis, substrate RNAs can be designed to identify components of the editing apparatus that associate with target transcripts. The proposed research should enhance understanding of other forms of RNA processing in the biological world, especially the mechanisms of tRNA, mRNA and rRNA modification and editing.