RNA EDITING IN PLANT ORGANELLES

植物细胞器中的 RNA 编辑

• 批准号: 6385864
• 负责人: MAUREEN REBECCA HANSON
• 金额: $ 29.73万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-05-01 至 2003-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6385864
• 关键词: RNA binding protein; chloroplasts; double stranded RNA; gene expression; genetic regulatory element; genetic translation; genetically modified plants; laboratory rabbit; mitochondria; nucleic acid sequence; nucleotides; plant genetics; plant proteins; polymerase chain reaction; posttranscriptional RNA processing; protein biosynthesis; tissue /cell culture; tobacco

RNA editing alters the predicted nuclear or organelle gene products from many organisms, ranging from protozoans to humans. This distinctive form of RNA processing gives organisms unique flexibility in producing messenger and structural RNAs and proteins from DNA genomes. For organelles of some plants and animals, RNA editing can be viewed as a correction mechanism for genes that otherwise could not be expressed or would encode aberrant products. RNA editing also sometimes serves to allow the production of proteins with diverse functions or properties from a single gene. In humans, transcripts of genes involved in lipid metabolism and nervous system function are among those that undergo base modifications. Fundamental aspects of base modification RNA editing will be uncovered by studies of C-to-U RNA editing in plastids of angiosperm plants. Unlike other organelles in which RNA editing occurs plastids can be transformed with deliberately altered editing sites to analyze the cis-acting elements necessary for catalysis and recognition of bases for modification. Mutant screens will identify nuclear or organelle genes which affect editing site recognition and editing efficiency. With information obtained from transformation analysis, substrate RNAs can be designed to identify components of the editing apparatus that associate with target transcripts. The proposed research should enhance understanding of other forms of RNA processing in the biological world, especially the mechanisms of tRNA, mRNA and rRNA modification and editing.

RNA编辑改变了预测的核或细胞器基因产物

项目成果

Fully edited and partially edited nad9 transcripts differ in size and both are associated with polysomes in potato mitochondria.

完全编辑和部分编辑的 nad9 转录本大小不同，并且都与马铃薯线粒体中的多核糖体相关。

• DOI: 10.1093/nar/24.7.1369
• 发表时间: 1996
• 期刊: Nucleic acids research
• 影响因子: 14.9
• 作者: Lu,B;Hanson,MR
• 通讯作者: Hanson,MR

Sequence elements critical for efficient RNA editing of a tobacco chloroplast transcript in vivo and in vitro.

• DOI: 10.1093/nar/gkl490
• 发表时间: 2006
• 期刊: Nucleic acids research
• 影响因子: 14.9
• 作者: Hayes ML;Reed ML;Hegeman CE;Hanson MR
• 通讯作者: Hanson MR

Expression of complementary RNA from chloroplast transgenes affects editing efficiency of transgene and endogenous chloroplast transcripts.

• DOI: 10.1093/nar/gki286
• 发表时间: 2005
• 期刊: NUCLEIC ACIDS RESEARCH
• 影响因子: 14.9
• 作者: Hegeman, CE;Halter, CP;Owens, TG;Hanson, MR
• 通讯作者: Hanson, MR

Protein polymorphism generated by differential RNA editing of a plant mitochondrial rps12 gene.

植物线粒体 rps12 基因差异 RNA 编辑产生的蛋白质多态性。

• DOI: 10.1128/mcb.16.4.1543
• 发表时间: 1996
• 期刊: Molecular and cellular biology
• 影响因子: 5.3
• 作者: Lu,B;Wilson,RK;Phreaner,CG;Mulligan,RM;Hanson,MR
• 通讯作者: Hanson,MR

High-level expression of a synthetic red-shifted GFP coding region incorporated into transgenic chloroplasts.

• DOI: 10.1046/j.1365-313x.2001.01088.x
• 发表时间: 2001-08
• 期刊: The Plant journal : for cell and molecular biology
• 作者: M. Reed;S. Wilson;C. Sutton;M. Hanson