HOUSING ENRICHMENT AND EXPERIMENTAL MANIPULATION

住房富集和实验操作

• 批准号: 6394704
• 负责人: DAVID M LAWSON
• 金额: $ 25.77万
• 依托单位: WAYNE STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-07-01 至 2003-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6394704
• 关键词: age difference; animal care; animal welfare research; blood pressure; body weight; catecholamines; corticosterone; estrus; gender difference; heart rate; housing; injection /infusion; isolation /deprivation; laboratory rat; postmortem; prolactin; psychophysiology; restraint; stress; telemetry

The long-range goal of this project is to conduct systematic studies on the effects of housing enrichment and common husbandry and experimental procedures on the normal cardiovascular and endocrine physiology of rats using state-of-the-art methodologies. It is anticipated that the results will greatly assist Institutional Animal Care and Use Committees in their determination of what procedures can or cannot be conducted in rodent housing areas and whether or not enrichment of the housing environment significantly reduces stress in rodents. The specific aims are: (1) To evaluate several levels of enrichment of the housing environment on distress (or lack of distress) in rats and (2) To evaluate the level of distress in animals undergoing common husbandry or experimental procedures or "witnessing" these procedures being done on other animals. These studies will be conducted on 3-, 6- or 12-month old male and female rats of four commonly used outbred strains obtained from commercial vendors. Animals will be "instrumented" with a radio-telemetry transmitter to continuously monitor arterial blood pressures and heart rate or with a chronic, indwelling venous catheter to obtain sequential blood samples for the determination of plasma levels of stress related hormones (catecholamines, corticosterone and prolactin). Additional variables (weight gain, food and water consumption and estrous cyclicity) will also be monitored to gauge the physiologic status of the rats. The housing conditions to be evaluated are: isolated (single) housing, paired (double) housing, isolated housing with "chew" and "climbing/hiding" "toys" and isolated housing with access to a very enriched environment for a short period each day. Adaptation to the environmental housing conditions will also be evaluated. Common husbandry and experimental manipulations to which animals are to be exposed or witness being done on other animals will include: removal to a new cage, hand restraint and a s.c. injection, placement in a rodent restrainer for a short period with a tail vein injection, witnessing the decapitation of other animals and witnessing a tissue harvest from other animals. Although this project is a descriptive one and not hypothesis driven, it should provide very practical and important results that can be used by local IACUCs as they review animal protocols and by national organizations such as the PHS, AAALAC, ILAR and USDA as they promulgate requirements, guidelines and regulations in this area.

该项目的长期目标是采用最先进的方法，系统地研究饲养条件的改善和共同饲养以及实验程序对大鼠正常心血管和内分泌生理的影响。 预计这些结果将极大地帮助机构动物护理和使用委员会确定在啮齿动物饲养区可以或不可以进行哪些程序，以及饲养环境的丰富是否显著降低啮齿动物的压力。 具体目标是：（1）评价饲养环境对大鼠痛苦（或无痛苦）的几种富集水平，以及（2）评价接受普通饲养或实验程序或“目睹”对其他动物进行这些程序的动物的痛苦水平。 这些研究将在3、6或12月龄雄性和雌性大鼠中进行，这些大鼠来自从商业供应商处获得的4种常用远交品系。 动物将“配备”无线电遥测发射器，以连续监测动脉血压和心率，或使用长期留置静脉导管，以连续采集血样，用于测定应激相关激素（儿茶酚胺、皮质酮和催乳素）的血浆水平。 还将监测其他变量（体重增加、摄食量和饮水量以及发情周期），以评估大鼠的生理状态。 待评价的饲养条件为：隔离（单人）饲养、成对（双人）饲养、带有“咀嚼”和“攀爬/隐藏”“玩具”的隔离饲养以及每天可在短时间内进入非常丰富的环境的隔离饲养。 还将评估对住房环境条件的适应情况。 动物暴露或见证对其他动物进行的常见饲养和实验操作包括：转移到新笼中、手部约束和s.c.注射，在啮齿动物限制器中放置短时间并进行尾静脉注射，见证其他动物的断头和见证从其他动物的组织收获。 虽然本项目是描述性的，而不是假设驱动的，但它应该提供非常实用和重要的结果，可供当地IACUC在审查动物方案时使用，也可供国家组织（如PHS、AAALAC、ILAR和USDA）在颁布该领域的要求、指南和法规时使用。