BIOACTIVE FORMS OF PROLACTIN: REGULATION OF SECRETION

拟议的研究涉及生物化学性质的问题， 催乳素生物活性形式的生理调节 哺乳期和外源性处理的大鼠。 此前的报告显示， 垂体中至少有三个催乳素“池”。 一个新 催乳素的合成池可以释放，大概是最迅速的 垂体中的任何形式，或转换为不 直接从催乳素释放，而是转化为 第二个存储池，可以从中进行释放。 这种转变 在哺乳期大鼠中有明显的过程，但在其他 生理条件。 第二个最有趣的特点是 储存形式的另一个问题是，它通常无法通过许多提取方法检测到。 具体的实验设计（1）本地化这些池与 不同的溶解和离心程序（2）来描述 在合成和释放期间控制这些池之间的移动 使用几种不同的动物模型，包括妊娠、哺乳和哺乳期， 类固醇治疗的大鼠（3），以确定是否有各种分子大小的 通过Sephadex凝胶过滤确定催乳素与这些池相关 和聚丙烯酰胺凝胶电泳技术（4），以确定什么 垂体催乳素的形式是生物活性的使用最近 淋巴瘤细胞生物测定法。

The proposed studies address the issues of the biochemical nature and physiologic regulation of biologically active forms of prolactin in lactating and extrogen treated rats. Previous reports suggest that there are at least three "pools" of prolactin in the pituitary. A newly synthesized pool of prolactin can be released, presumably most rapidly of any form in the pituitary, or converted to a storage pool which is not directly released from the lactotroph but instead is transformed to a second storage pool from which releases can occur. This transformation process is marked in the lactating rat but unresolved during other physiological conditions. The most interesting feature of the second storage form is that it is often undetectable by many extraction methods. specific experiments are designed (1) to localize these pools with different solubiliztion and centrifugation procedures (2) to describe the controls over movement between these pools during synthesis and release using several different animal models including pregnant, lactating and steroid treated rats (3) to determine if various molecular sized of prolactin relate to these pools as determined by Sephadex gel filtration and polyacrylamide gel electrophoretic techniques (4) to determine what forms of pituitary prolactin are biologically active using the recently developed lymphoma cell bioassay.