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Engineering the bacterial cellulose producer K. rhaeticus iGEM to create new cellulose materials

对细菌纤维素生产者 K. rhaeticus iGEM 进行工程改造，以制造新的纤维素材料

基本信息

批准号：
1846146
负责人：
金额：
--
依托单位：
Imperial College London
依托单位国家：
英国
项目类别：
Studentship
财政年份：
2016
资助国家：
英国
起止时间：
2016 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=studentship-1846146
关键词：
Engineering bacterial cellulose producer K.

项目摘要

Bacterial cellulose is an ultra-pure carbohydrate polymer, which is biocompatible with a high tensile strength and water holding potential. Synthetic biology holds the potential to engineer the bacteria, which produce cellulose to create new biopolymers with different functional characteristics for practical applications. One of the most efficient producers of bacterial cellulose is the Gram-negative bacterium Komagataeibacter rhaeticus (k. rhaeticus IGEM), isolated from a kombucha tea SCOBY. Past work on this bacterium led to the development of the K. rhaeticus synthetic biology toolkit v1.0, which provided the basic tools to regulate cellulose production with signal molecules such as AHL. We hope to expand upon this tool kit to provide alternative methods of regulating cellulose production, as well as providing methods to alter the morphology, spatial arrangement and functional properties of bacterial cellulose. We plan to add to the toolkit, CRISPR/Cas9 based genomic modification; CRISPRi gene regulation and trans-membrane photoreceptor proteins sensors. These will allow for the precise control of cellulose synthesis. Following the expansion of the toolkit, we plan to use these tools to alter the properties of bacterial cellulose and create patterned and functionalised composite materials.

细菌纤维素是一种超纯的碳水化合物聚合物，其具有高拉伸强度和持水性，具有生物相容性。合成生物学具有工程细菌的潜力，这些细菌产生纤维素以创造具有不同功能特性的新生物聚合物用于实际应用。细菌纤维素最有效的生产者之一是革兰氏阴性细菌Komagataeibacter rhaeticus（k. rhaeticus IGEM），分离自红茶菌茶SCOBY。过去对这种细菌的研究导致了K。rhaeticus synthetic biology toolkit v1.0，它提供了用信号分子如阿勒调节纤维素生产的基本工具。我们希望扩展这个工具包，以提供调节纤维素生产的替代方法，以及提供改变细菌纤维素的形态、空间排列和功能特性的方法。我们计划在工具包中添加基于CRISPR/Cas9的基因组修饰; CRISPRi基因调控和跨膜感光蛋白传感器。这些将允许精确控制纤维素合成。随着工具包的扩展，我们计划使用这些工具来改变细菌纤维素的特性，并创建图案化和功能化的复合材料。