GOLDENSEAL(HYDRASTIS CANADENSIS)REMEDY FOR ORAL DISEASES

金印（加拿大水螅）治疗口腔疾病

• 批准号: 6226269
• 负责人: Christine D Wu
• 金额: $ 17.34万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-15 至 2003-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6226269
• 关键词: alternative medicine; antibiotics; chemical structure function; disease /disorder prevention /control; drug discovery /isolation; human therapy evaluation; medicinal plants; oral health

DESCRIPTION (APPLICANT'S ABSTRACT): Complementary and alternative medicine (CAM) has recently gained popularity with the American public. Research validating CAM has focused mainly on the treatment and prevention of systemic medical diseases while less attention has been paid to oral diseases. Oral diseases including dental caries and periodontal disease, are a major cause of loss of work and school days. Chemical and mechanical means have been used to control dental plaque bacteria, the etiologic agent of caries and periodontal disease. However, none of the available agents is ideal and frequently cause adverse effects. This justifies further search and development of alternative agents from natural sources that are safe and effective. The North American plant, Hydrastis canadensis L. (Ranunculaceae), known commercially as "Goldenseal," has been used for centuries as an antiseptic to treat skin disorders and as an antidiarrheal, antiseptic, astringent, hemostatic, and vasoconstrictor agent. Goldenseal is one of the major phytomedicines ("herbal remedies") sold in health food stores and pharmacies in the U.S. Several mouthrinses and toothpastes containing Goldenseal are available on the market. Although claims have been made by the manufactures regarding its ability to fight gum diseases and prevent caries, no scientific data is available to substantiate these claims. The goal of the proposed research is to evaluate the potential of Goldenseal as a remedy in prevention and treatment of oral diseases and to maintain oral health. It is hypothesized that antimicrobial compounds that are safe for humans can be identified from H. canadensis. These compounds may have potential as dental prophylactic/therapeutic agents and may also serve as lead compounds for the subsequent design and synthesis of new agents that are even more effective than the existing ones. The Specific Aims of this study are: SA1: To isolate and identify active antimicrobial compounds from H. canadensis by activity-guided fractionation and characterization; SA2: To determine antimicrobial activity of the purified compounds against cariogenic and periodontal pathogens; SA3: To investigate mixtures of purified antimicrobial compounds from H. canadensis for synergistic antimicrobial activities; SA4: To correlate bioactivity of various commercially available Goldenseal-containing oral hygiene products with levels of active alkaloids identified in SA2. The proposed research is innovative in that it represents collaboration between an oral microbiologist and a natural product chemist that will assure the speedy discovery of novel or known active compounds from Goldenseal and will provide scientific explanation as to the remedy's efficacy. It will also serve as a model system for the evaluation of existing herbal remedies for their oral health related claims. This application of CAM research will help to achieve better oral health and oral disease prevention, one of the to priority areas of focus specified b the U.S. Public Health Service in "Health People 2000."

描述（申请人的摘要）：补充和替代药物 （CAM）最近在美国公众中广受欢迎。研究 验证CAM主要集中于全身治疗和预防 医疗疾病虽然对口腔疾病的关注较少。口服 包括龋齿和牙周疾病在内的疾病是 失去工作和上学的日子。化学和机械手段已用于 对照牙菌斑细菌，龋齿和牙周的病因学剂 疾病。但是，没有一个可用的代理人是理想的，并且经常引起 不利影响。这证明了替代方案的进一步搜索和发展 来自自然来源的代理商安全有效。 北美植物，Hydrastis Canadensis L.（Ranunculaceae），已知 在商业上作为“戈德米”已被用作数百年来 治疗皮肤疾病，并作为抗动脉，防腐剂，与收敛剂 止血和血管收缩剂。金esheal是主要的 植物医学（“草药疗法”）在保健食品商店和药房中出售 美国几个嘴骨和牙膏含有金eseal 在市场上可用。尽管制造商已经提出了索赔 关于它与牙龈疾病和预防龋齿的能力，没有科学 数据可用于证实这些主张。提议的目标 研究是为了评估黄金化作为预防补救措施的潜力 和口腔疾病的治疗并维持口腔健康。它是假设的 可以从H中鉴定出对人类安全的抗菌化合物。 加拿大。这些化合物可能具有牙齿的潜力 预防性/治疗剂，也可以用作铅化合物 随后的设计和合成的新代理人比 现有的。这项研究的具体目的是：SA1：隔离和 通过活性引导，鉴定来自加拿大h。H.canadensis的活性抗菌化合物 分馏和表征； SA2：确定抗菌活性 纯化的化合物针对牙科和牙周病原体； SA3：到 研究纯化的抗菌化合物的混合物 协同抗菌活性； SA4：与各种生物活性相关 具有水平的市售的含戈金斯的口腔卫生产品 在SA2中鉴定的活性生物碱。 拟议的研究具有创新性，因为它代表 口服微生物学家和天然产品化学家，将确保 快速发现戈尔卡利的新颖或已知活性化合物，并将 就补救措施的功效提供科学解释。它也将服务 作为评估其口服现有草药疗法的模型系统 与健康有关的主张。这种CAM研究的应用将有助于实现 更好的口腔健康和预防口腔疾病，这是优先领域之一 重点指定的B美国公共卫生服务在“健康人2000”中。