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Detection and Monitoring of Podocyturia Using RT-QPCR

使用 RT-QPCR 检测和监测足细胞尿

基本信息

批准号：
6406373
负责人：
Kevin D McBryde
金额：
$ 5.96万
依托单位：
UNIVERSITY OF MICHIGAN AT ANN ARBOR
依托单位国家：
美国
项目类别：
财政年份：
2001
资助国家：
美国
起止时间：
2001-08-14 至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/6406373
关键词：
chronic renal failure diabetic nephropathy diagnosis design /evaluation disease /disorder proneness /risk glomerulonephritis human subject insulin dependent diabetes mellitus kidney cell kidney disorder diagnosis patient oriented research polymerase chain reaction postdoctoral investigator proteinuria urinalysis

项目摘要

The loss of podocytes from the glomerular basement membrane (GBM) represents the loss of an irreplaceable component of the glomerular filtration barrier that predisposes regions of bare GBM to form adhesions to the parietal epithelium of Bowman's capsule. These adhesions are the beginnings of synechiae, which progress to the histopathology of focal segmental glomerulosclerosis characteristic of many renal disorders culminating in chronic renal insufficiency (CRI) or end stage renal disease (ESRD). In the adult population, diabetes mellitus accounts for nearly 40% of the cases of ESRD, whereas in the pediatric population, glomerulonephritis accounts for 30% of cases of ESRD. The proposed research will quantitatively demonstrate podocyte loss into the urine utilizing a non-invasive fluorescent reverse-transcriptase quantitative polymerase chain reaction (RT-QPCR). Longitudinally collected urine samples from case patients with glomerulonephropathies and Type I diabetes mellitus will be tested after informed consent has been obtained. Fluorescent RT-QPCR is performed using an intricate series of primers and probes designed for the podocyte-specific protein nephrin. The goals of this project are to longitudinally monitor the case patients' clinical courses and laboratory tests and to correlate these with their quantitative loss of podocytes in the urine. Additionally, the project will quantify urinary podocyte loss before and after initiation of medical therapy, and seek to identify those patients who continue to lose podocytes and are at greatest risk for the development of proteinuria, CRI or ESRD.

肾小球基底膜 (GBM) 足细胞的丧失代表肾小球滤过屏障不可替代成分的丧失，该成分使裸露的 GBM 区域易于与鲍曼囊壁上皮形成粘连。这些粘连是粘连的开始，粘连发展为局灶节段性肾小球硬化的组织病理学特征，是许多肾脏疾病的特征，最终导致慢性肾功能不全（CRI）或终末期肾病（ESRD）。在成年人群中，糖尿病占 ESRD 病例的近 40%，而在儿童群体中，肾小球肾炎占 ESRD 病例的 30%。拟议的研究将利用非侵入性荧光逆转录酶定量聚合酶链反应（RT-QPCR）定量证明足细胞进入尿液的损失。纵向收集的肾小球肾病合并I型糖尿病患者的尿液样本将在获得知情同意后进行检测。使用专为足细胞特异性蛋白去氧肾上腺素设计的一系列复杂的引物和探针进行荧光 RT-QPCR。该项目的目标是纵向监测病例患者的临床过程和实验室检查，并将其与尿液中足细胞的定量损失相关联。此外，该项目还将量化开始药物治疗之前和之后的尿足细胞丢失情况，并寻求识别那些持续丢失足细胞且发生蛋白尿、CRI 或 ESRD 风险最大的患者。

项目成果

期刊论文数量（3）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Clearance of amino acids by hemodialysis in argininosuccinate synthetase deficiency.

精氨酸琥珀酸合成酶缺乏症通过血液透析清除氨基酸。

DOI：
10.1016/j.jpeds.2004.01.037
发表时间：
2004
期刊：
The Journal of pediatrics.
影响因子：
0
作者：
McBryde,KevinD;Kudelka,TimothyL;Kershaw,DavidB;Brophy,PatrickD;Gardner,JohnJ;Smoyer,WilliamE
通讯作者：
Smoyer,WilliamE

Hyperosmolar solutions in continuous renal replacement therapy for hyperosmolar acute renal failure: a preliminary report.

高渗性急性肾衰竭连续肾脏替代治疗中的高渗性解决方案：初步报告。