Core--Pathology and animal

病理学/动物核心将为计划项目提供显微镜细胞和组织的统一处理和染色。核心将提供来自4个项目的光和透射电子显微镜(EM)的专家免疫组织化学。更重要的是，它将提供一位在炎症和修复领域经验丰富的病理学家，他可以比较和整合所有模型中的病理结果。所采用的大多数免疫病理学方法对所有项目都是通用的，因此核心实验室将提供统一的方法和解释。纸巾的中央处理将节省成本、质量控制和项目结果之间的可比性。由一位病理学家对所有组织学结果的持续审查将确保立即认识到来自不同项目的病理结果的共同和独特方面。核心的动物部分将为项目1、3和4中使用的完整的心肌缺血/再灌注动物模型提供准备和手术。这些模型将包括在小鼠、大鼠和狗身上进行的急性和/或慢性研究。每一种模型都是为特定的科学目的而选择的，并将由一名对每种模型都有经验的生理学家进行监督。

The Pathology/Animal Core will provide the Program Project uniform processing and staining of cells and tissues of microscopy. The Core will offer expert immunohistochemistry on light and transmission electron microscopy (EM) from the 4 Projects. More importantly, it will provide an experienced pathologist in the field of inflammation and repair, who can compare and integrate the pathological findings in all of the models. Most of the immunopathological methods employed are common to all of the projects so that a Core laboratory will provide uniform methods and interpretation. The central processing of tissue will result in cost savings, quality control and comparability between the results of the projects. The continual review of all of the histological findings by a single pathologist will ensure that both common and unique aspects of pathological results from different projects are appreciated immediately. The Animal portion of the Core will provide preparation and surgery for the intact animal models of myocardial ischemia/reperfusion used in Projects 1, 3, and 4. These models will include acute and/or chronic studies in mice, rats, and dogs. Each model has been chosen for a specific scientific purpose and will be supervised by a physiologists who had previous experience with each.