Histology Core

组织学核心

• 批准号: 9086201
• 负责人: William M Baldwin
• 金额: $ 26.71万
• 依托单位: CLEVELAND CLINIC LERNER COM-CWRU
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-06-01 至 2020-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9086201
• 关键词: Animal Model; Antibodies; Computer software; Controlled Study; Cost Savings; Documentation; Experimental Designs; Histology; Histopathology; Image Analysis; Individual; Manuscripts; Mediating; Methods; Microscopic; Pathology; Photography; Preparation; Quality Control; Reagent; Research Personnel; Resources; Staining method; Tissue Stains; Tissues; Transplantation; abstracting; clinically relevant; design; digital; kidney allograft; novel; quality assurance; quantitative imaging; response

Project Summary / Abstract This core centralizes the pathology resources for all of the projects of the PPG. This results in cost savings, increased quality control and maximal comparability between the results of the projects. Importantly, new stains and methods are developed as required by novel findings that evolve in individual Project. These new stains and methods are then provided to every Project in a unified manner. Key aims of the Core are to provide: 1) expert advise in immunohistological aspects of experimental design to optimize results; 2) uniform histochemical and immunohistochemical staining of tissue sections; 3) pathological interpretation of tissue sections; 4) documentation of the pathology by high quality digital microscopic photography; 5) preparation of figures for manuscripts; 6) software for quantitative image analysis; 7) develop and evaluate additional reagents for new targets in response to novel findings In addition, this Core is responsible for ordering, quality assurance and control studies of all antibodies and reagents for use in the pathology methods. New reagents are developed and evaluated as requested by investigators in the 3 projects. Histopathology is central to all 3 projects. Each animal model is designed to answer clinically relevant questions. To that end, every transplant is evaluated relative to the Banff criteria for antibody mediated rejection (AMR).

项目总结/摘要 该核心集中了PPG所有项目的病理学资源。这导致成本节约， 加强质量控制和项目结果之间的最大可比性。重要的是，新 染色剂和方法是根据个别项目中出现的新发现而开发的。这些新 然后以统一的方式向每个项目提供染色剂和方法。 核心方案的主要目标是： 1)在免疫组织学方面的专家建议实验设计，以优化结果; 2)组织切片的均匀组织化学和免疫组织化学染色; 3)组织切片的病理学解释; 4)通过高质量数字显微摄影记录病理学; 5)为手稿准备数字; 6)定量图像分析软件; 7)针对新发现开发和评估新靶点的额外试剂 此外，该中心还负责所有抗体的订购、质量保证和控制研究， 用于病理学方法的试剂。根据以下要求开发和评价新试剂： 三个项目的调查员。 历史学是所有3个项目的核心。每种动物模型的设计都是为了回答临床相关问题 问题.为此，相对于抗体介导的Banff标准评估每个移植物。 拒绝（AMR）。