Histology Core

项目摘要/摘要 这一核心集中了PPG所有项目的病理资源。这导致了成本节约， 加强质量控制和项目结果之间的最大可比性。重要的是，新的 根据在个人项目中发展的新发现的要求来开发染色和方法。这些新的 然后，将染色和方法以统一的方式提供给每个项目。 核心的主要目标是提供： 1)在实验设计的免疫组织学方面提供专家建议，以优化结果； 2)组织切片组织化学和免疫组织化学染色均一； 3)组织切片的病理解释； 4)用高质量的数字显微照相记录病理； (五)稿件数字的准备； 6)定量图像分析软件； 7)开发和评估针对新发现的新靶点的额外试剂 此外，该中心负责所有抗体的订购、质量保证和控制研究 病理学方法中使用的试剂。应以下要求开发和评估新试剂 3个项目中的调查人员。 组织病理学是所有三个项目的核心。每一种动物模型都是为回答临床相关问题而设计的 问题。为此，根据Banff抗体介导的标准对每例移植进行评估。 拒绝(AMR)。

Project Summary / Abstract This core centralizes the pathology resources for all of the projects of the PPG. This results in cost savings, increased quality control and maximal comparability between the results of the projects. Importantly, new stains and methods are developed as required by novel findings that evolve in individual Project. These new stains and methods are then provided to every Project in a unified manner. Key aims of the Core are to provide: 1) expert advise in immunohistological aspects of experimental design to optimize results; 2) uniform histochemical and immunohistochemical staining of tissue sections; 3) pathological interpretation of tissue sections; 4) documentation of the pathology by high quality digital microscopic photography; 5) preparation of figures for manuscripts; 6) software for quantitative image analysis; 7) develop and evaluate additional reagents for new targets in response to novel findings In addition, this Core is responsible for ordering, quality assurance and control studies of all antibodies and reagents for use in the pathology methods. New reagents are developed and evaluated as requested by investigators in the 3 projects. Histopathology is central to all 3 projects. Each animal model is designed to answer clinically relevant questions. To that end, every transplant is evaluated relative to the Banff criteria for antibody mediated rejection (AMR).