Imaging force-dependent cytoskeletal dynamics in tumour cells

肿瘤细胞中成像力依赖性细胞骨架动力学

• 批准号: 1903433
• 金额: --
• 依托单位: King's College London
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2017
• 资助国家: 英国
• 起止时间: 2017 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1903433
• 关键词: Imaging; force; dependent; cytoskeletal; dynamics

Actin-based cell membrane protrusions are known to drive growth and movement of cancer cells but the mechanisms initiating these processes are poorly understood. Tumour microenvironments exhibit complex and heterogeneous forces that are emerging as important cues in controlling cytoskeletal dynamics. However, analysis of cytoskeletal dynamics in 3D matrices has proven extremely challenging due to limitations in imaging approaches. This project will combine the development of advanced microscopy with computational modeling to define the impact of force on cytoskeletal dynamics and tumour cell invasion in 3D environments. This will provide novel tools and insight into studying cancer cell behavior within more physiologically relevant settings.

已知基于肌动蛋白的细胞膜突起驱动癌细胞的生长和运动，但启动这些过程的机制知之甚少。肿瘤微环境表现出复杂和异质的力量，正在成为控制细胞骨架动力学的重要线索。然而，由于成像方法的限制，在3D矩阵中分析细胞骨架动力学已被证明是极具挑战性的。该项目将联合收割机的先进显微镜的发展与计算建模，以确定在三维环境中的细胞骨架动力学和肿瘤细胞侵袭力的影响。这将为在更生理相关的环境中研究癌细胞行为提供新的工具和见解。