Imaging force-dependent cytoskeletal dynamics in tumour cells using single-molecule localisation microscopy

使用单分子定位显微镜对肿瘤细胞中力依赖性细胞骨架动力学进行成像

• 批准号: 2339463
• 金额: --
• 依托单位: King's College London
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2339463
• 关键词: Imaging; force; dependent; cytoskeletal; dynamics

Single-molecule localisation microscopy (SMLM) separates fluorophore emissions in time to create a tenfold increase in spatial resolution along all three axes. However, long acquisition times make live-cell imaging difficult. These can only be shortened by acquiring 'high-density' data, which increases the risk of generating artificial structures in the final image - a risk that is further increased when imaging in three dimensions. The Haar Wavelet Kernel software (HAWK) is designed to remove artefacts during high-density imaging, and we are using the technology to develop a HAWK Method of Assessment (HAWKMAN) for SMLM images. Using a variety of point-spread function asymmetries, we have also been running evaluations of HAWK's propensity for handling different types of 3D data. Future work will see these technologies applied to live-cell, 3D SMLM imaging using selective plane illumination microscopy to study cancer cell motility mechanics in 3D environments over short timescales.

单分子定位显微镜（SMLM）会及时分离荧光团排放，以创造沿所有三个轴的空间分辨率提高十倍。但是，漫长的获取时间使实时成像变得困难。这些只能通过获取“高密度”数据来缩短这些，从而增加在最终图像中产生人工结构的风险 - 在三个维度成像时，这种风险会进一步增加。 HAAR小波内核软件（HAWK）旨在在高密度成像过程中删除伪影，我们正在使用该技术来开发SMLM图像的Hawk评估方法（Hawkman）。使用多种点传播函数不对称，我们还一直在评估Hawk处理不同类型的3D数据的倾向。未来的工作将使这些技术使用选择性平面照明显微镜在3D环境中研究癌细胞运动力学的3D SMLM成像。