CORE--NMR

近年来，多维核磁共振波谱研究取得了很大进展。 蛋白质三维结构的可能测定 (大小小于25kD)和溶液中的多肽。此外，侧面- 链和主干动力学测量允许确定位置- 关于灵活性领域的具体信息。的内部运动 蛋白质，如单个氨基酸残基的旋转以及 残基的协调节段性运动，广泛存在于 不同的时间尺度和随机热运动以及 与特定配体和或蛋白质的相互作用。这些波动是 被认为在生物功能中非常重要，包括 酶催化、变构作用、蛋白质-蛋白质相互作用和配体 有约束力的。目前对这些动议的细节的了解还很零碎。 核心主任已经使用这些方法来研究折叠和动力学 抹香鲸肌红蛋白。此外，处长还聘请了 异核滤过法检测蛋白质与配体的结合 互动。她将继续使用这些方法来调查 蛋白质动力学在具有生物重要性的蛋白质系统中的作用。我们 已经组装了一个核心设施，可以获得这项技术 致该计划项目的所有调查人员。 1)提供蛋白质核磁共振测量方面的技术专长。 2)帮助设计新的针对特定目标的实验 具有生物学重要性的问题 3)为研究人员提供核磁共振波谱使用方面的培训。 核心设施的组织是为了提供最大的协作潜力 对所有调查人员来说，并最大限度地利用最新的- 用ART技术解决具有生物重要性的问题。

In recent years, advances in multi-dimensional NMR spectroscopy have made possible the determination of the three-dimensional structures of proteins (of sizes less than 25 kD) and peptides in solution. In addition, side- chain and backbone dynamics measurements allow the determination of site- specific information on regions of flexibility. Internal motions of proteins, such as rotations of individual amino acid residues as well as concerted segmental motions of groups of residues, occur on widely different time scales and result from random thermal motions as well as interactions with specific ligands and or proteins. These fluctuations are recognized as being very important in biological functions, including enzyme catalysis, allosterism, protein-protein interactions and ligand binding. Current knowledge of the details of these motions is fragmentary. The Core Director has used these methods to study the folding and dynamics of sperm whale myoglobin. In addition, the Director has employed heteronuclear filtering methods to probe protein-ligand binding interactions. She will continue to use these methods to investigate the role of protein dynamics in protein systems of biological importance. We have assembled a core facility which will give access to this technology to all the Investigators of the Program Project. 1) to provide technical expertise in protein NMR measurements. 2) to provide aid in the design of new experiments to target specific questions of biological importance 3) to provide training to investigators in the use of NMR spectroscopy. The core facility is organized to offer maximum collaborative potential for all investigators, and to maximize the use of the latest state-of-the- art techniques to address problems of biological importance.