TIME RESOLVED SMALL ANGLE XRAY SCATTERING OF REFOLD OF SPERM WHALE APOMYOGLOBIN

抹香鲸无肌红蛋白重折叠的时间分辨小角X射线散射

• 批准号: 6437703
• 负责人: PATRICIA A JENNINGS
• 金额: $ 14.32万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-03-01 至 2002-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6437703
• 关键词: bioimaging /biomedical imaging; biomaterials; biomedical resource; proteins; radiography; structural biology

We propose to perform stopped-flow time-resolved small-angle x-ray scattering studies of the refolding of sperm whale apomyoglobin under the same conditions used in a recent folding study employing stopped-flow circular dichroism and pulse labeling NMR techniques. We want to obtain further information regarding the size and time-scale of formation of a kinetic molten globule intermediate on the apolmyoglobin refolding pathway. The insights provided by these experiments will help to further interpret the NMR results, and should lead to a clearer understanding of the folding mechanism of apomyoglobin in particular and of proteins in general.

我们建议进行停流时间分辨小角度x射线 散射法研究抹香鲸脱辅基肌红蛋白的折叠 在最近的折叠研究中使用了相同的条件， 停流圆二色性和脉冲标记NMR技术。 我们 我想获得更多的信息，关于规模和时间尺度 形成一个动力学熔融球中间体上的 脱辅基肌红蛋白重折叠途径 这些提供的见解 实验将有助于进一步解释核磁共振结果，并应 从而更清楚地了解 特别是脱辅基肌红蛋白和一般蛋白质。