CORE A: NMR CORE

蛋白激酶和磷酸酶的正确细胞内靶向赋予了这些酶的特异性，在 部分，通过将它们放置在靠近它们喜欢的底物的地方。此外，针对 酶可能会改变蛋白质的活性，因为结构和动力学与生物密切相关 活动。要了解这种活动的物理基础，需要了解蛋白质的性质 在其自然环境中，处于各种自由和束缚状态。构象变化可能是由微妙的 由粗大波动引起的运动，如肽键的旋转，如折叠 蛋白质，或来自多蛋白质中结构域相对于彼此的相对取向的变化 很复杂。 核磁共振核心在下一个授权期的目标是： 我继续支持高分辨率多核核磁共振方法 和 为膜相关蛋白的样品制备和分析提供培训和专业知识 使用溶液和固体核磁共振方法相结合的结构域。

The correct intracellular targeting of protein kinases and phosphatases confers specificity to the enzymes, in part, by placing them in close proximity to their preferred substrates. In addition, the targeting of the enzymes may alter the activity of the protein as structure and dynamics are intimately linked with biological activity. Understanding the physical basis for this activity requires knowledge of the properties of the protein in its native environment, in various free and bound states. Conformational changes may result from subtle movements, such as the rotation of a peptide bond, from gross fluctuations, such as the folding of the protein, or from changes in the relative orientation of domains with respect to one another in a multiprotein complex. The goals for the NMR Core in the next granting period are to: I Provide continued support for high-resolution multinuclear NMR methods and Provide training and expertise for sample preparation and analysis of membrane associated proteins and domains with a combination of solution and solid-state NMR methods.