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TIME RESOLVED SMALL ANGLE XRAY SCATTERING OF REFOLD OF SPERM WHALE APOMYOGLOBIN

TIME RESOLVED SMALL ANGLE XRAY SCATTERING OF REFOLD OF SPERM WHALE APOMYOGLOBIN
抹香鲸无肌红蛋白重折叠的时间分辨小角X射线散射
批准号：
6658752
负责人：
PATRICIA A JENNINGS
金额：
$14.32万
依托单位：
STANFORD UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2002
资助国家：
美国
项目状态：
已结题
起止时间：
2002-03-01 至 2003-02-28
来源：
https://reporter.nih.gov/project-details/6658752
关键词：
bioimaging /biomedical imaging biomaterials biomedical resource proteins radiography structural biology

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英文摘要
We propose to perform stopped-flow time-resolved small-angle x-ray scattering studies of the refolding of sperm whale apomyoglobin under the same conditions used in a recent folding study employing stopped-flow circular dichroism and pulse labeling NMR techniques. We want to obtain further information regarding the size and time-scale of formation of a kinetic molten globule intermediate on the apolmyoglobin refolding pathway. The insights provided by these experiments will help to further interpret the NMR results, and should lead to a clearer understanding of the folding mechanism of apomyoglobin in particular and of proteins in general.
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