REBASE--THE RESTRICTION ENZYME DATABASE

该项目涉及到一个关于限制性内切核酸酶及其相关甲基化酶的连贯和全面的信息数据库的维护和分发。 REBASE自1975年以来一直存在，目前在SYBASE下以关系格式实现。 数据库中的信息来自已发表的文献，以及研究者在发表前或未发表时直接提交的信息。 大约11%的条目是通过REBASE以外的其他方式发布的。 这些信息通过科学文献的正式出版物和各种电子格式在全世界传播。 因此，每月都张贴更新，并通过自动电子邮件列表向350多个网站分发选定的文件。 此外，还可通过匿名FTP或通过万维网获取信息。在每月维护和更新的文件中，有许多被广泛使用的计算机程序（如GCG、MacVector、DNA Strider等）使用的专用文件。这些文件随时可用，包括有关全部特性及其商业可用性的最新信息。 通过REBASE获得的信息是所有计划切割和/或重新连接DNA实验的研究人员的关键。它是生物技术实践的独特资源，其内容直接或间接地被研究卫生保健社区的许多部门使用。

This project is concerned with the maintenance and distribution of a cohesive and comprehensive database of information about restriction endonucleases and their associated methylases. REBASE has been in existence since 1975 and is currently implemented in a relational format under SYBASE. Information enters the database both from the published literature and by direct submission from the investigators either prior to publication or instead of publication. Approximately 11% of the entries are unpublished other than through REBASE. The information is disseminated throughout the world both by formal publication in the scientific literature and in a variety of electronic formats. Thus, monthly updates are posted and selected files distributed through an automatic email list to more than 350 sites. In addition, the information is available by anonymous FTP or through the World Wide Web. Among the files maintained and updated monthly are specialized ones that are used by many of the more widely used computer programs, such as GCG, MacVector, DNA Strider etc. These files are ready to use and include up-to-date information about the full range of specificities and their commercial availability. The information available through REBASE is key to all investigators planning experiments to cleave and/or religate DNA. It is a unique resource for the practice of Biotechnology and its contents are used directly or indirectly by many sections of the research health care community.